Alanine scanning mutagenesis

Duchesnes CE, Murphy PM, Williams TJ, Pease JE. Alanine scanning mutagenesis of the chemokine receptor CCR3 reveals distinct extracellular residues involved in recognition of the eotaxin family of chemokines. Mol Immunol 2006 43(8) 1221-1231. 

Pons, J. Rajpal, A. Kirsch, J.F., Energetic analysis of an antibody/antigen interface alanine scanning mutagenesis and double mutant cycles on the HyHEL-10/lysozyme interaction, Prot. Sci. 1999, 8, 958-968. 

In addition to the C-terminus, several other regions of Ga have been implicated in receptor binding by a variety of experimental approaches (Fig. 4). Residues in the a4//16 loop contribute to the binding surface as demonstrated by alanine-scanning mutagenesis (Onrust et al, 1997), studies with chimeric Ga proteins (Bae et al., 1997, 1999), sequence analysis of conserved amino acids in Ga subclasses (Lichtarge et al., 1996), chemical cross-linking (Cai et al, 2001), and protection from tryptic proteolysis (Mazzoni and Hamm, 1996). The C-terminal 11 amino acids of Gat and... 

Harlow GR, Halpert JR (1997) Alanine-scanning mutagenesis of a putative substrate recognition site in human cytochrome P4503A4. J Biol Chem 272, 5396-5402. 

COMPUTATIONAL DETERMINATION OF THE RELATIVE FREE ENERGY OF BINDING - APPLICATION TO ALANINE SCANNING MUTAGENESIS... 

The purpose of this review is not an exhaustive description of all the methods in the literature to calculate relative binding differences, and therefore we are going to present only those, which have been applied to computational mutagenesis, especially alanine scanning mutagenesis. 

Scheme 6-2. Resume of the Methodological Approach for Computational Alanine Scanning Mutagenesis...

Moreira IS, Fernandes PA, Ramos MJ (2006) Computational alanine scanning mutagenesis - an improved methodological approach, J Comput Chem, In Press... 

Gunningham, B. C., and Wells, J. A. (1989). High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science 244, 1081-1085. 

Auger, G. A., Pease, J. E., Shen, X., Xanthou, G., and Barker, M. D. (2002). Alanine scanning mutagenesis of CCR3 reveals that the three intracellular loops are essential for functional receptor expression. Eur. J. Immunol. 32, 1052-1058. 

Yang M, Chen X, Militello K, Hoffman R, Fernandez B, Baumann C, Gollnick P. Alanine-scanning mutagenesis of Bacillus subtilis... 

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