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Oligonucleotide directed codon mutagenesis

Oligonucleotide directed codon mutagenesis + selection E. coli [83]... [Pg.1561]

For mutagenesis to introduce the stop codons, the protocols in Subheadings Oligonucleotide Phosphorylation with T4 Polynucleotide Kinase, Annealing of the Oligonucleotide to the Template, and step 1 of Enzymatic Synthesis of CCC-dsDNA can be scaled down by a factor of ten. The reaction product can be used directly to transform E. coli using any standard procedure. [Pg.270]

See other pages where Oligonucleotide directed codon mutagenesis is mentioned: [Pg.198]    [Pg.206]    [Pg.1500]    [Pg.461]    [Pg.835]    [Pg.5]    [Pg.259]    [Pg.587]    [Pg.356]    [Pg.566]    [Pg.141]    [Pg.353]    [Pg.350]    [Pg.163]    [Pg.363]    [Pg.2414]    [Pg.356]    [Pg.58]    [Pg.59]    [Pg.62]    [Pg.301]    [Pg.335]    [Pg.828]    [Pg.831]    [Pg.38]    [Pg.38]    [Pg.38]    [Pg.105]    [Pg.31]    [Pg.162]    [Pg.202]    [Pg.364]    [Pg.366]    [Pg.273]    [Pg.253]   
See also in sourсe #XX -- [ Pg.125 ]



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Mutagenesis oligonucleotide-directed

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