Chorismate mutase mutagenesis

The results of these selection experiments are mechanistically significant insofar as they support a critical role for a cation in the mechanism of chorismate mutase. No active catalysts were found that lacked a cation in the vicinity of the substrate s ether oxygen. The conclusion that a cation is crucial for high chorismate mutase activity can thus be made with much greater confidence than would have been possible following a conventional mutagenesis experiment in which only single substitutions were considered [66]. Of course, we cannot exclude the possibility that other, equally effective... 

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