Polymerase chain reaction , random mutagenesis method

Leung, D.W., Chen, E. and Goeddel, D.V. (1989) A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique, 1, 11-15. 

The method for random mutagenesis of genes using error-prone polymerase chain reaction (PCR) was adapted from previous reports (34, 35). An error rate of approximately 0.5% should be expected using this protocol. For a single yeast library of random mutants of approximately 105 clones, you should prepare enough reactions to yield 50—80 Llg error-prone amplified insert (between 3 and 8 reactions). 

A useful method for the generation of molecular diversity starting from a given gene is to use a method of random mutagenesis by error-prone polymerase chain reaction (error-prone PCR) (Figure 10.8). Using error-prone PCR, the mutation rate must be carefully tuned - beneficial mutations are rare but deleterious mutations are common. If the mutation... 

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