Mutagenesis screens

However, this process in itself is not yet directed evolution, because only one cycle of mutagenesis/screening is involved. Isolated cases involving at least two such cycles began to appear, being the first cases of true directed evolution of enzymes (29c), but it was not until the period of 1993—1997 that the idea of directed... 

Kool J, Berns A (2009) High-throughput insertional mutagenesis screens in mice to identify oncogenic networks. Nat Rev Cancer 9 389-399... 

The identification of putative mammalian clock genes by both homology and mutagenesis screening is described elsewhere in this volume. It represents an enormous achievement, and already it is possible to speak of the new circadian biology in which molecular genetics can be used to understand and re-interpret more classical, black-box descriptions of mammalian circadian behaviour. A key component of this analysis is to understand the behaviour of the clock genes, and... 

Moreover, their housing is less space- and cost-intensive, which predestines them for large mutagenesis screens, selective breeding and quantitative trait loci studies. Although rats are principally indispensable for behavioural experiments, mice will clearly dominate the experimental analysis of learning and memory for the next decade. 

These selected sequences are then amplified and the cycle of mutagenesis, screening, and amplification is repeated until proteins with the desired property or function are found. 

Smith, F. M. et al (2004). Dissecting the EphA3/ephrin-A5 interactions using a novel functional mutagenesis screen./. Biol. Chem. 279, 9522-9531. 

Subtilisin E Activity in organic solvents - 170-fold increase in 60% di-methylformamide Random mutagenesis + screening B. subtilis [14,197]... 

Subtilisin BPN Stability in the absence of Ca2+ 1000-fo u iiiCrcaSc 111 half-lift Loop removed i cassette mutagenesis + screening B. subtilis [198]... 

Hydantoinase Enantioselectivity + total activity Inverted enantioselectivity, 5 x increase in total activity Random mutagenesis + saturation mutagenesis + screening E. coli [65]... 

B. stearothermophilus amidase Increase expression in E. Coli Increase expression (23 x) Random mutagenesis + screening E. coli [210]... 

Horseradish peroxidase Increase activity/ expression in S. cerevisiae Increase total activity 40 x Random mutagenesis + screening S. cereivisiae [156]... 

Random mutagenesis + saturation mutagenesis + screening B. subtilis [64]... 

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