Salmonella typhimurium mutagenesis

Barrueco, C. de la Pena, E. (1988). Mutagenic evaluation of the pesticides Captan, Folpet, Dkhlofluanid and related compounds with the mutants TA 102 and TA 104 of Salmonella typhimurium. Mutagenesis, 3, 467-80. 

Wilcox, R, Naidoo, A., Wedd, D.J. and Gatehouse, D.G. (1990). Comparison of Salmonella Typhimurium TA102 with Escherichia coli WP2 tester strains. Mutagenesis 5 285-291. 

Three primary tests are incorporated in the health effects area. The microbial mutagenesis assay is based on the property of selected Salmonella typhimurium mutants to revert from a histidine requiring state to prototrophy due to exposre to various classes of mutagens. The test can detect nanogram quantities of mutagens and has been adapted to mimic some mammalian metabolic processes by the addition of a mammalian liver microsomal fraction. The test is used as a primary screen to determine the mutagenic activity of complex mixtures or component fractions. 

Desmutagenic activity. Fresh plant juice, on agar plate at a concentration of 0.5 mL/ disc, was inactive on Salmonella typhimurium TA98 L Homogenate of the fresh root, at a concentration of 100 pL/disc on agar plate, was active on Salmonella typhimurium TA98 and TAIOO vs l,4-dinitro-2-methyl pyrrole mutagenesis ". 

Antimutagenic activity. Water extract of the dried gum, on agar plate at a concentration of 2 mg/plate, was inactive on SalmO nella typhimurium TAIOO vs aflatoxin Bl-induced mutagenesis and a concentration of 10 mg/plate, was inactive on SalmO nella typhimurium TA98 " . Asafoetida, on agar plate at a dose of 0.5 (Xg/plate was active on Salmonella typhimurium TA98 and... 

Roldan-Arjona. T. Pueyo, C. (1993) Mutagenic and lethal effects of halogenated methanes in the Ara test of Salmonella typhimurium. quantitative relationship with chemical reactivity. Mutagenesis. 8. 127-131... 

Kerklaan, P., Bouter, S. Mohn, G. (1983) Isolationof a mutant of Salmonella typhimurium strain TAI 535 with decreased levels of glutathione (GSH-) primary characterization and chemical mutagenesis studies. Mutat. Res.. 122, 257-266... 

Gdggelmann, W., Bauchinger, M., Kulka, U. Schmid, E. (1988) Genotoxicity of 2-nitropropanc and 1-nitropropane in Salmonella typhimurium and human lymphocytes. Mutagenesis, 3, 137-140... 

Roldan-Aijona, T., Garcia-Pedrajas, D., Luque-Romero, F.L., Hera, C. Pueyo. C. (1991) An association between mutagenicity of the Ara test of Salmonella typhimurium and carcinogenicity in rodents for 16 halogenated aliphatic hydrocarbons. Mutagenesis, 6, 199-205... 

Mutagenesis induced in Salmonella typhimurium by 2-fluorenamine and other chemical carcinogens was inhibited by low levels of retinol and other retinoids when carcinogen activation was carried out by rat liver microsomes. In contrast, low levels of retinoids enhanced mutagenesis when carcinogen activation was mediated by whole liver homogenates. There was no effect of the provitamin 3-carotene in this test system. 

The Ames test is used to screen a wide variety of chemicals for potential carcinogenicity or as potential cancer chemotherapeutic agents. The test enables a large number of compounds to be screened rapidly by examining their ability to induce mutagenesis in several specially constructed bacterial mutants derived from Salmonella typhimurium. 

Santamaria, L., Tatea, F. and Bianchi, L. 1987b. Rosmarinus officinalis extract inhibits as antioxidant mutagenesis by 8-methoxypsoralen (8-MOP) and benzo(a)pyrene (BP) in Salmonella typhimurium. Med. Biol. Env. 15 92-101. 

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