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Mutagenesis definition

While the results of this work are encouraging, it is clear that the structural definition of mutant proteins of this type is critical to development of rational interpretation of the results if for no other reason than that the structural perturbation introduced is presumably greater than for simple point mutations. Moreover, it would be particularly interesting to compare the functional properties of mutants compared in this manner in assays involving protein-protein reactions relevant to the species of cytochrome c on which the mutagenesis is based. For example, comparison of the activities of wild-type yeast cytochrome c with that of a loop-insertion mutant modelled on a photosynthetic cytochrome c in the reaction with the photosynthetic reaction center could help define the structural elements involved in the cytochrome c binding domain for the reaction center. [Pg.149]

Prasad VR, Goff SP. Linker insertion mutagenesis of the human immunodeficiency virus transcriptase expressed in bacteria definition of the minimal polymerase domain. Proc Natl Acad Sci USA 1989 86 3104-3108. [Pg.688]

However, most public concern does not center around death or other acute intoxication symptoms, but rather those chronic injuries which we term as irreversible. These are carcinogenesis (cancer), teratogenesis (birth defects), or mutagenesis (genetic defects). There have been three good studies involving the ability of 2,4-D to cause cancer. The conclusion by the authors of these three studies is that there is no evidence that 2,4-D causes cancer. However, the study design was such that they were not adequate to prove that 2,4-D could not cause cancer, and as a result, further cancer studies were required by the EPA which should provide a definitive answer. [Pg.340]

Even though there is a possibility that the apical methionine ligand may control the reduction potential, the definite role still remains ambiguous (75). Synthetic studies with model compounds, in conjunction with protein work by site-directed mutagenesis, should be undertaken to further address this point. [Pg.20]

It is possible to alter amino-acid residues to see if this perturbs the EPR signal, e.g. by iodination of tyrosine residues [142]. Recently site-directed mutagenesis has been used extensively for this purpose. Whilst this can yield definitive negative results it is unlikely to lead to clear positive identification of a... [Pg.88]


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Mutagenesis

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