Combining Site-directed Mutagenesis with Chemical Modification

Combining Site-directed Mutagenesis with Chemical Modification... 

One example of the combined use of site-directed mutagenesis with chemical modification has been provided by the work of Bowler et al. with yeast iso-l-cytochrome c [15]. These workers introduced a His residue at position 62 to provide a site for attachment of a pentammineruthenium complex. Introduction of a second redox-active metal center to the protein at this position permitted... 

Combining site-directed mutagenesis strategies with chemical modification is a popular tool in both enzyme engineering and mechanistic studies. This has often been applied to the subtilisin from Bacillus lentus (SBL), or savinase. Subtilisins are... 

If necessary, these combining sites may be equipped with an appropriately positioned catalytic amino acid side chain or a cofactor, which may be introduced either directly by site-directed mutagenesis or indirectly by chemical modification. Antibodies bind molecules ranging in size from about 6 to 34 A with association constants of M , in other words, binding is extremely strong. As with... 

---