Mutagenesis analysis

The results of site-directed mutagenesis analysis of zinc ligands of higher plant p-carbonic anhydrase and of P. purpureum carbonic anhydrase have confirmed that zinc is essential for catalysis. X-ray fine structure data indicated that a water molecule is hydrogen bonded to the zinc-ligated Asp-151 and Asp-405. The water molecule is not directly coordinated to the zinc atom. A possible catalytic mechanism of C02 hydration cycle (211) has been proposed as given in Scheme 10. 

Fig. 3 Evaluation of structures from the Protein Data Bank to identify and assess helical interfaces in protein-protein (HIPP) interactions. The helical interfaces were segregated based on binding interfaces and computational alanine scanning mutagenesis analysis. AAGavg > 2 kcal/mol AAGavg = 1-2 kcal/mol (Reprinted with permission from Jochim and Arora [50], Copyright (2010) American Chemical Society)...

Bardy SL, Ng SYM, Carnegie DS et al. (2005) Site-directed mutagenesis analysis of amino acids critical for activity of the type I signal peptidase of the archaeon Methanococcus voltae. JBacteriol 187(3) 1188-1191... 

Nagy, A. and Rossant, J. (1996) Targeted mutagenesis Analysis of phenotype without germ line transmission. J. Clin. Invest. 97,1360-1365. 

Sequence aligments of hydratase/isomerase proteins show that Glul65 of the mitochondrial short-chain isomerase and Glul64 of the mitochondrial hydratase from rat are conserved throughout the family." It has been shown by site-directed mutagenesis analysis that the replacement of these residues with glutamine leads to reduced activity. The results of crystallization studies of hydratase 1 are in line with this observation since the data showed that Glu 164 provides an a-proton during hydration reactions. ... 

Mutagenesis analysis of sequences in type B HA analogous to the binding pocket helix found in H3 HA and HEF suggests that this structure has a different... 

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