Mammalian mutagenesis

Safrole was positive in the following short-term tests for mutagenesis mammalian cell transformation in culture, Rabin s test of degranulation of rough endoplasmic reticulum... 

A. W. Hsie, J. P. O NeiU, and V. K. McEUieney, eds.. Mammalian Cell Mutagenesis The Maturation of Test Systems, Banbury Report No. 2, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1979. 

Szklarz GD, Halpert JR. Use of homology modeling in conjunction with site-directed mutagenesis for analysis of structure-function relationships of mammalian cytochromes P450. Life Sci 1997 61 2507-20. 

Moraes, E.C., Keyse, S.M. and Tyrrell, R.M. (1990). Mutagenesis by hydrogen peroxide treatment of mammalian cells a molecular analysis. Carcinogenesis 11, 283-293. 

Chen, L. and Dixon, K., Analysis of repair and mutagenesis of chromium induced DNA damage in yeast mammalian cells and transgenic mice, Environmental, Health Perspective, 106, 1027-1032, 1998. 

Bryant The work published on the mammalian insulin receptor shows that glycosylation is required for function. There is a mutagenesis study showing that there is a particular Asp residue, without which the receptor is expressed on the membrane but doesn t function (Leconte et al 1994). In other studies the biosynthesis of the oligosaccharides has been inhibited, and this has the same kind of effect (Podskalny et al 1984). 

Evans, H.H. 1990. Ionizing radiation mutagenesis in mammalian cells. Pages 211-219 in UCLA Symposia Colloquium. Ionizing Radiation Damage to DNA Molecular Aspects. Wiley-Liss, New York. 

Albanese, R. (1987). Mammalian male germ cell cytogenetics. Mutagenesis 2 79-85. 

Clive, D. (1987). Historical overview of the mouse lymphoma TK+/, mutagenicity assay. In Mammalian Cell Mutagenesis, (Moore, M.M., Demarini, D.M., De Serres, F.J. and Tindall, K.R., Eds.). Banbury Report 28. Cold Spring Harbor Laboratory, NY, pp. 25-36. 

Fox, M. (1981). Some quantitative aspects of the response of mammalian in vitro to induced mutagenesis. In Cancer Biology Reviews (Marchelonis, J.J. and Hanna, M.G., Eds.). Vol. 3, Marcel Dekker, New York, pp. 23-62. 

Friedman MA, Staub J. 1976. Inhibition of mouse testicular DNA synthesis by mutagens and carcinogens as a potential simple mammalian assay for mutagenesis. Mutat Res 37 67-76. 

Three primary tests are incorporated in the health effects area. The microbial mutagenesis assay is based on the property of selected Salmonella typhimurium mutants to revert from a histidine requiring state to prototrophy due to exposre to various classes of mutagens. The test can detect nanogram quantities of mutagens and has been adapted to mimic some mammalian metabolic processes by the addition of a mammalian liver microsomal fraction. The test is used as a primary screen to determine the mutagenic activity of complex mixtures or component fractions. 

Mutagenesis Tetracycline and oxytetracycline have produced positive mutagenic results in mammalian cell assays in vitro. 

Domanski, T. L. and Halpert, J. R. (2001) Analysis of mammalian cytochrome P450 structure and function by site-directed mutagenesis. Curr. Drug. M. 2, 117-137. 

Naegeli H. Roadblocks and detours during DNA replication mechanisms of mutagenesis in mammalian cells. Bioessays 1994 16 557-564. 

Hsie AW, Johnson NP, Couch DB, et al. 1979. Quantitative mammalian cell mutagenesis and a preliminary study of the mutagenic potential of metallic compoimds. In Kharasch N, ed. Trace metals in health and disease. New York, NY Raven Press, 55-69. 

The identification of putative mammalian clock genes by both homology and mutagenesis screening is described elsewhere in this volume. It represents an enormous achievement, and already it is possible to speak of the new circadian biology in which molecular genetics can be used to understand and re-interpret more classical, black-box descriptions of mammalian circadian behaviour. A key component of this analysis is to understand the behaviour of the clock genes, and... 

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