Protein recombinant

E. Zerovnik, R. Jerala, L. Rroon-Zitko, R. H. Pain, and V. Turk, Intermediates in denaturation of a small globular protein, recombinant human Stefin B, J. Biol. Chem, 13, 9041 (1992). 

Figure 4.5. Integrative and excisive X phage recombination pathways. Integration is catalyzed by the X Int protein in a reaction that also requires the E. coli IHF protein. Recombination occurs within a common core sequence of 15 base pairs. The excision reaction requires the X Xis protein in addition to Int and IHF.

Udit, A.K., Silberg, J.J. and Sieber, V. (2003) Sequence homology-independent protein recombination (SHIPREC). Methods in Molecular Biology (Clifton, NJ), 231, 153-163. 

Vaccines made with recombinant proteins marshal an immune response to specific viruses to treat or prevent disease. Vaccines to prevent infection with the liver-damaging hepatitis viruses (Twinrix , Recombivax HB , and Engerix-B ) include a recombinant version of a hepatitis virus B protein. Recombinant hepatitis B protein is also included in some combination vaccines given to children. Comvax also includes a recombinant flu virus protein. 

Proteins, recombinant DNA ORIGIN alteplase anistreplase tenecteplase... 

For the purpose of synthesizing flavor peptides or proteins in large scale, we developed "protein recombination method" and "enzymatic synthesis using chemically modified enzyme". "Protein recombination method" was applied to the synthesis of C-terminal portion of p-casein and its analog. Chymotrypsin was chemically modified by Z-DSP in aqueous solution. It was stable for organic solvents. Using this modified enzyme, we succeeded in the synfiiesis of Inverted-Aspartame-Type Sweetener "Ac-Phe-Lys-OH" in one step. 

Today, it is well-known that peptides or proteins exhibit various kinds of taste. Our group has been researching on the relationship between taste and structure of peptides, BPIa (Bitter peptide la, Arg-Gly-Pro-Pro-Phe-Ile-Val) (7 as a bitter peptide, Om-p-Ala-HCl (OBA), Om-Tau-HCl as salty peptides(2j, and "Inverted-Aspartame-Type Sweetener" (Ac-Phe-Lys-OH) as a sweet peptide(5). The relationship between taste and chemical structure was partly made clear. Since commercial demand for these flavor peptides is increasing, we need to develop new synthetic methods which can prepare these peptides in large scale. We developed the following two methods (1) protein recombination method as a chemical method, (2) enzymatic synthesis using chemically modified enzyme as a biochemical method. 

Flavor notes and products of lipid oxidation in cooked meats, 85,86/ Flavor peptides convenient synthesis, 149-156 enzymatic synthesis, 151,154-156 protein recombination synthetic method, 149... 

N. Dasovich, T.D. Sweeney, S.J. Shire, C.C. Hsn, and Y.E Maa, Effect of mannitol crystallization on the stability and aerosol performance of a spray-dried pharmaceutical protein, recombinant humanized anti-IgE monoclonal antibody. J Pharm Sci, 1998. 87(11) 1406-11. 

IL-2, rDNA/Seragen [BIO] A-L-Methionyl-387-L-histidine-388-L-alanuie-l-388-toxin (Corynebacterium diphtheriae strain Cl) (388-2 ) protein with 2-133-interleukin 2 (human clone pTIL2-21a) [CAS] Interleukin-2 Fusion Protein [SY] Interleukin-2/diphtheria toxin fusion protein, recombinant [SY]... 

A number of protocols will be described here that have been used successfully with both rat (Y3 and IR983F) and mouse (SP2/0) myelomas to generate MAbs to cellular proteins, recombinant proteins, or peptides based on cDNA sequences. Successful hybridoma production relies on the ability to ... 

Plates coated with purified soluble antigen. As an alternative to step 7 above, and providing purified soluble antigen is available, 96-well PS or polyvinyl chloride (PVC) plates can be coated with protein, recombinant protein, peptide, or peptide conjugated to a carrier protein Plates are coated as follows... 

Sequence Homology-Independent Protein Recombination (SHIPREC) no requirement for sequence homology requires size separation of DNA prior to screening only one crossover per gene Sieber, 2001... 

This segment includes a detailed example of capture and recovery of intracellular protein (recombinant exotoxin A from E. coli) and key parameters and conditions for obtaining other products. 

Costantino, H. R., Langer, R., and Klibanov, A. (1995), Aggregation of a lyophilized pharmaceutical protein, recombinant human albumin effect of moisture and stabilization excipients, BioTechnology, 13,493-496. 

The formation of beads is a two-step process based on the cold gelation of whey proteins in the presence of divalent cations, such as Ca2+ [67], Briefly, the whey protein isolate (WPI) solution (10% w/v in deionized water) was (i) adjusted at pH 7 to favor the apparition of negative charges implied in ionic bounds with Ca2+ ions and (ii) heated (80 °C, 45 min) to denaturate the proteins. Recombinant cells in the beginning of their stationnary growth phase were suspended in a sterile solution of... 

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