Homolog-scanning mutagenesis

Other, perhaps alternative, approaches that could be used for epitope mapping include homolog-scanning mutagenesis (5), which could also be a means of complementing the secondary structure predictions. This involves the synthesis of an array of small peptides that completely span the protein... 

Site-directed mutagenesis, cysteine-scanning accessibility methods, high field NMR, homology modeling, and continued development of structure-activity relationships will no doubt lead to better and better models of the monoamine transporters. These approaches are having the greatest impact on studies of uptake inhibitors, rather than studies of substrates. 

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