Mutagenesis deletion

Dale GE, Kostrewa D, GseU B, Stieger M, D Arcy A. 1999. Crystal engineering deletion mutagenesis of the 24 kDa fragment of the DNA Gyrase B subunit from Staphylococcus Aureus. Acta Cryst D55 1626-1629. 

Woemer AM, Marcus-Sekura CJ. Characterization of a DNA binding domain in the C-terminus of HIV-l integrase by deletion mutagenesis. Nucl Acids Res 1993 21 3507-3511. 

Gross, C H. and Rohrmann, G. F (1990) Mapping unprocessed epitopes using deletion mutagenesis of gene fusions. Biotechniques 8, 196-202. 

E., Probing the ligand binding domain of the GluR2 receptor by proteolysis and deletion mutagenesis defines domain boundaries and yields a crystallizable construct. Protein Sci. 1998, 7, 2623-2630. 

Like modular PKSs, peptide synthetases also epimerize some substrates and/or intermediates. For example, the starter substrate amino acid of cyclosporin A is D-Ala. Racemization of alanine is not catalyzed by an integrated subunit of cyclosporin A synthetase, but by alanine racemase. This is a separate, pyridoxal phosphate-dependent enzyme [ 193]. In contrast, Grsl and Tycl covalently activate L-Phe as a thioester and subsequently epimerize the amino acid [194]. D-Phe is the only epimer accepted as a substrate for dipeptide formation by Grs2 and Tyc2 [195, 196]. No racemization activity is detected in a pantetheine-deficient mutant of Grsl [197]. Deletion mutagenesis pointed to the requirement of the COOH-terminal part of the module for epimerizing L-Phe to D-Phe [180]. In contrast, the biosynthesis of actinomycin D, a bicyclic chromo-pentapeptide lactone (Fig. 10), involves formation of the dipeptide 6-MHA (methylanthranilic acid)-L-Thr-L-Val prior to epimerization of the L-Val exten-... 

Cloning of human cGI-PDEfrvm HEL cells and platelets, expression in yeast and localization of catalytic domain by deletion mutagenesis... 

Cammarata KV and Schmidt GW (1992) In vitro reconstitution of a light-harvesting gene product Deletion mutagenesis and analyses ofpigment binding. Biochemistry 31 2779-2789... 

Pakrasi HB, Diner BA, Williams JGK et al. Deletion mutagenesis of the cytochrome b559 protein inactivates the reaction center of photosystem II. Plant Cell 1989 1 591-597. 

DELETION MUTAGENESIS OF PSBF. THE GENE FOR THE p-SUBUNTT OF CYTOCHROME B559 IN SYNECHQCYSTIS PCC 6803. 

Deletion Mutagenesis of PSBF the Gene for the p-Subunit of Cytochrome B559 in Synechocystis PCC 6803 367... 

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