Dopamine site-directed mutagenesis

Chen, N., Vaughan, R. A., and Reith, M. E. (2001) The role of conserved tryptophan and acidic residues in the human dopamine transporter as characterized by site-directed mutagenesis. J. Neuro chem. 77,1116-1127. 

Numerous site-directed mutagenesis studies have provided a conclusive picture for the molecular interactions between the receptor-activating biogenic amines (e.g. serotonin, epinephrine, dopamine) and their receptors [23-27] a highly conserved aspartate residue in transmembrane (TM) helix TM3 (Asp 3.32 according to the Ballosteros-Weinstein nomenclature) [28], conserved serine residues in TM5 (e.g. 

Fig. 6.3 Amino acid residues required for receptor desensitization and internalization the dopamine Dj receptor example. The substitution of Glu or Thr by Ala results in desensitiza-tion-deficient mutants of the dopamine receptor, but they are stUl able to internalize to some extent. Phosphorylation sites in a 12-amino acid stretch of the distal carboxyl tail ( Thr to Thr and Thr) may be involved in internalization of the receptor. The variant constructs (substitutions by Ala) were generated by site-directed mutagenesis and expressed in cultured Chinese hamster ovary (CHO) cells (122)...

Daubner SC, Lauriano C, Haycock JW, Fitzpatrick PF (1992) Site-directed mutagenesis of serine 40 of rat tyrosine hydroxylase. Effects of dopamine and cAMP-dependent phosphorylation on enzyme activity. J Biol Chem 267 12639 16... 

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