Site-directed mutagenesis steady-state kinetics

Lu, H., Tsai, S.-C., Khosla, C. and Cane, D.E. (2002) Expression, site-directed mutagenesis, and steady state kinetic analysis of the terminal thioesterase domain of the methymycin/picromycin polyketide synthase. Biochemistry, 41, 12590-12597. 

The reader who has read both Chapter 1 of Volume II and this chapter will have a broad introduction to the subject of steady-state kinetics. Although the tools of the kineticist have become more powerful and sophisticated, the number of people well versed in their use has not grown in parallel fashion. This is probably because textbooks still do not cover the subject adequately moreover, the only useful information in print other than the primary literature is in chapters such as this one or other reviews. We hope this chapter will make it easier for enzymologists to learn these methods, which they now need more than ever to characterize the mechanisms of all of the altered enzymes that site-directed mutagenesis can provide. 

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