Transposons mutagenesis

Vanstockem,M., Michiels, K., Vanderleyden, J. and Van Gool, A., 1987 Transposon mutagenesis of Azospirillum hrasilense and Azospirillum lipoferum, physical analysis of Tn5 and lr 5-moh insertion mutants. Appl. Environ. Microbiol. 53 410-415. 

Don RH, AJ Wightman, HH Knackmuss, KN Timmis (1985) Transposon mutagenesis and cloning analysis of the pathways of degradation of 2,4-dichlorophenoxyacetic acid and 3-chlorobenzoate in Alcaligenes eutrophus JMP134 (pJP4). J Bacterial 161 85-90. 

Mutants impaired in the synthesis and accumulation of PHAs have been obtained from conventional chemical mutagenesis or transposon mutagenesis from many bacteria. Mutant PHB4 from R. eutropha strain H16 [130] and mutant GpP104 from P. putida strain KT2442 [36] are most probably those mutants which were mostly used, although the genotypes of these mutants were never analyzed. 

Sharma M. 2005. Transposon mutagenesis of gene involved in limonin degradation in Pseudomonas putida. M.C. diss., Patiala. 

B. Fodor, G. Rakhely, A. T. Kovdcs, K. L. Kovacs (2001) Transposon mutagenesis in purple sulfur photosynthetic bacteria Identification of hypF, encoding a protein capable to process [NiFe] hydrogenases in a, p and y subdivision of proteobacteria. Appl. Environ. Microbiol., 67 2476-2483... 

Vertes AA, Asai Y, Kobayashi M, et al. 1994. Transposon mutagenesis of coryneform bacteria. Mol Gen Genet 245 397 05. 

Sedgwick, S. G, Nguyen thi Man, Ellis, J M., Crowne, H, and Morris, G. E (1991) Rapid mapping by transposon mutagenesis of epitopes on the muscular dystrophy protein, dystrophin. Nucleic Acids Res 19, 5889-5894. 

CA Hutchison, SN Peterson, SR Gill, RT Cline, O White, CM Fraser, HO Smith, JC Venter. Global transposon mutagenesis and a minimal Mycoplasma genome. Science 286 2165-2169, 1999. 

Wang FFFF, Fraser MJ, Cary LC (1989), Transposon mutagenesis of baculoviruses analysis of TFP3 lepidopteran transposon insertions at the FP locus of nuclear polyhedrosis viruses, Gene 81 97-108. 

Acinetobacter baylyi ADP1 499 Transposon mutagenesis de Berardinis et al., 2008... 

Petzke L, Luzhetskyy A (2009) In vivo Tn5-based Transposon Mutagenesis of Streptomy-cetes. Appl Microbiol Biotechnol 83 979... 

A mutant defective in the chemotaxis toward 4-hydroxybenzoate in P. putida was obtained by Tn5 transposon mutagenesis, and allowed identification of a gene cluster pcaRKF that encodes enzymes of the 3,4-dihydroxybenzoate branch of the (i-ketoadi pate pathway pcaR encodes regulatory genes, pcaK the thiolase in the last step of the pathway, and pcaF is involved in transport of, and chemotaxis toward 4-hydroxybenzoate (Harwood et al. 1994). 

The most important adhesion molecule for native surfaces is S. epidermidis autolysin AtlE. This was identified by transposon mutagenesis in a mutant defective in attachment to polystyrene but which could still attach to glass [31, 32], An AtlE-defective mutant was attenuated in virulence in a rat central venous catheter infection model [33], and in fact AtlE seems to have a dual role in attachment, as it also binds specifically to the ECM protein vitronectin [32, 34],... 

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