Iterative saturated mutagenesis

Recently, a method called iterative saturation mutagenesis (ISM) has been proposed and experimentally implemented, which appears to be exceptionally efficient [23,24],... 

Figure 2.3 Schematic illustration of Iterative Saturation Mutagenesis (ISM) involving (as an example) four randomization sites A, B, C and D [23,24].

Reetz, M.T., Carballeira, J.D. and Vogel, A. (2006) Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability. Angewandte Chemie (International Edition in English), 45, 7745-7751. 

Fig. 2 Iterative saturation mutagenesis (ISM) employing four sites A, B, C, and D, each site in a given upward pathway being visited only once [48, 49]...

Bougioukou, D.J., KiHe, S., Taglieber, A., and Reetz, M.T. (2009) Directed evolution of an enantioselective enoate reductase testing the utility of iterative saturation mutagenesis. Adv. Synth. Catal., 351, 3287-3305. 

Acevedo-Rocha, C., MUe, S., and Reetz, M. T. (2013) Iterative saturation mutagenesis in protein engineering. Methods Mol. Biol., accepted. 

Reelz, M.T. and Carballeira, J.D. (2007) Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes. Nat. Protoc., 2, 891—903. 

TABLE 8.2 Selected EH Variants Evolved toward Higher Enantioconvergence using Iterative Saturation Mutagenesis (Including Desymmetrization of meso-Epoxides)... 

Reetz, M.T., Prasad, S., Carballeira, J.D., Gumulya, Y. and Bocola, M. (2010) Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity rigorous comparison with traditional methods. /. Am. Chem. Soc., 132,9144r-9152. 

P450py from Sphingomonas sp. HXN-200 Iterative saturation mutagenesis HPLC Activities toward p-substituted styrene increased up to 3-fold [33,134]... 

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