Mutagenesis mammalian assays

Friedman MA, Staub J. 1976. Inhibition of mouse testicular DNA synthesis by mutagens and carcinogens as a potential simple mammalian assay for mutagenesis. Mutat Res 37 67-76. 

Clive, D. (1987). Historical overview of the mouse lymphoma TK+/, mutagenicity assay. In Mammalian Cell Mutagenesis, (Moore, M.M., Demarini, D.M., De Serres, F.J. and Tindall, K.R., Eds.). Banbury Report 28. Cold Spring Harbor Laboratory, NY, pp. 25-36. 

Three primary tests are incorporated in the health effects area. The microbial mutagenesis assay is based on the property of selected Salmonella typhimurium mutants to revert from a histidine requiring state to prototrophy due to exposre to various classes of mutagens. The test can detect nanogram quantities of mutagens and has been adapted to mimic some mammalian metabolic processes by the addition of a mammalian liver microsomal fraction. The test is used as a primary screen to determine the mutagenic activity of complex mixtures or component fractions. 

Mutagenesis Tetracycline and oxytetracycline have produced positive mutagenic results in mammalian cell assays in vitro. 

Amacher, D.E. Paillet, S.C., and Turner, G.N. (1979b). Utility of the mouse lymphoma L5178Y/TK assay for the detection of chemical mutagens, in Banbury Report 2, Mammalian Cell Mutagenesis The Maturation of Tkst Systems, HsiE, A.W., O Neill, J.R, and McElheny, V.K, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York). 

Dean, B.J., Anderson, D. Sram, R.J. (1981) Mutagenicity of selected chemicals in the mammalian dominant lethal assay in comparative chemical mutagenesis. Environ. Sci. Res., 24,487-538... 

Mohn, G.R. Zeeland, A.A. (1985) Quantitative comparative mutagenesis in bacteria, mammalian cells, and animal-mediated assays. A convenient way of estimating genotoxic activity in vivo Mutat. Res., 150, 159-175... 

The adverse effect of many chemicals depends on their conversion to reactive electrophilic intermediates by mono-oxygenases.157 283 Most cell lines currently used for mutagenesis studies are not capable of these chemical conversions. Therefore, inclusion of a metabolic activation system in a test system is essential. In mutagenesis assays with mammalian cells, three metabolic activation systems have been used ... 

Metabolic activation systems—such as microsome-, cell-, and host-mediated assays—have been included in mammalian cell mutagenesis systems. Microsome-mediated assays have been used to detect many chemicals, including nitrosamines, polycyclic hydrocarbons, aflatoxins, and vinyl chloride. Cell-mediated assays seem to be a better indicator of in vivo metabolic pathways. Microsome-mediated assays seem suitable for general screening of chemicals, and cell-mediated assays are more valuable in the assessment of data. 

Mutagenesis Assays in Cultured Mammalian Cells 96 DNA Repair as a Predictive Indicator of... 

Dichlorvos has been shown to be positive in the Ames assay and in other bacterial and mammalian cell mutagenesis assays. 

O Neill JP and Hsie AW (1979) The CHO/HGPRT mutation assay experimental procedure. In Hsie AW, O Neill JP, and McElhenny VK (eds.) Mammalian Cell Mutagenesis The Maturation of Test Systems, Banbury Report No. 2, pp. 55-70, 311-318, 407-420. Cold Spring Harbor, NY Cold Spring Harbor Press. 

Sun F, Betzendahl I, Shen Y, Cortvrindt R, Smitz J, Eichenlaub-Ritter U (2004) Preantral follicle culture as a novel in vitro assay in reproductive toxicology testing in mammalian oocytes. Mutagenesis 19 13-25... 

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