Codon-level mutagenesis

Oligonucleotide-mediated mutagenesis was used to change the histidine codon at L173 (CAC) to a leucine codon (CTC). Photosynthetic growth assays indicate that His - Leu does not grow photosynthetically. The absorption spectrum of chromatophores from this mutant suggests that reduced levels of RCs are stably assembled in the membrane, (data not shown). RCs were purified from chromatophore membranes of His ... 

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