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Combinatorial multiple-cassette mutagenesis CMCM

Finally, the region of accessible protein sequence space was extended by developing a modified version of Stemmer s combinatorial multiple-cassette mutagenesis (CMCM)... [Pg.30]

The starting point for further exploration of protein sequence space was the conjecture that Stemmer s method of Combinatorial Multiple Cassette Mutagenesis (CMCM) [74] can be applied in appropriately modified form. It is a special type of DNA-shuffling which can be used to generate mutant gene libraries in which cassettes composed of random or defined sequences and the wild-type are incorporated randomly. CMCM had been developed for use in the area of functional antibodies [74],... [Pg.263]

Figure 2.9 Schematic summary of the directed evolution of enantioselective lipase variants originating from the WT PAL used as catalysts in the hydrolytic kinetic resolution of ester rac-1. CMCM = Combinatorial multiple-cassette mutagenesis [8c,22],... Figure 2.9 Schematic summary of the directed evolution of enantioselective lipase variants originating from the WT PAL used as catalysts in the hydrolytic kinetic resolution of ester rac-1. CMCM = Combinatorial multiple-cassette mutagenesis [8c,22],...
See other pages where Combinatorial multiple-cassette mutagenesis CMCM is mentioned: [Pg.34]    [Pg.83]    [Pg.34]    [Pg.83]    [Pg.1]    [Pg.83]   
See also in sourсe #XX -- [ Pg.30 ]

See also in sourсe #XX -- [ Pg.34 ]



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Cassettes

Combinatorial multiple-cassette mutagenesis

Multiple cassette mutagenesis

Mutagenesis

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