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Engineering metabolic

Metabolic manipulation can be achieved either by over-expression or knockout of key enzymes to increase or decrease the accumulation of particular end-products, or to divert existing pathways to create new products. However, the rational engineering of alkaloid [Pg.130]


Within the diastereomeric switch sequences, the corresponding trans-diols become accessible either using a Mitsunobu inversion or a reversible Diels-Alder cyclization as key reaction step [249,250]. This synthetic strategy is complementary to an approach involving metabolic engineering of E. coli via the chorismate/ isochorismate pathway [251]. [Pg.260]

Steinbuchel, A. and Lutke-Eversloh, T. 2003. Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochemical Engineering Journal 16 81-96. [Pg.39]

It is important that chemical engineers master an understanding of metabolic engineering, which uses genetically modified or selected organisms to manipulate the biochemical pathways in a cell to produce a new product, to eliminate unwanted reactions, or to increase the yield of a desired product. Mathematical models have the potential to enable major advances in metabolic control. An excellent example of industrial application of metabolic engineering is the DuPont process for the conversion of com sugar into 1,3-propanediol,... [Pg.930]

DELLAPENNA D (2001) Plant metabolic engineering . Plant Physiol, 125, 160-63. [Pg.275]

DHARMAPURI S, ROSATI C, PALLARA P, AQUILANI R, BOUVIER F, CAMARA B and GIULIANO G (2002) Metabolic engineering of xanthophyll content in tomato fruits , FEBSLett, 519, 30-34. [Pg.275]

GIULIANO G, AQUiLANi R and DHARMAPURi s (2000) Metabolic engineering of plant carotenoids , Trends Plant Sci, 5, 406-9. [Pg.276]

MISAWA N and shimada h (1998) Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts , J Biotechnol, 59, 169-81. [Pg.277]

While metabolic engineers traditionally sought the rate-limiting enzyme to unlock flow through a pathway, now they understand that there may be many points of control and feedback with the metabolic network, and seek to empirically determine the dynamics of the interactions between rate controllers and other factors. For example, the sizes of metabolic precursor pools and the catabolism or sequestration of products affect accumulation as well as flux through the pathway. [Pg.356]

UDP-GTase may be associated with membrane transporters and is a key component of pigment accumulation for the metabolic engineer to consider. [Pg.373]

Experiments in directed breeding have been carried out in bacteria and are proving grounds for metabolic engineering of pigment accumulations in plants. Experi-... [Pg.379]

Plant use is less biotechnologically advanced and fundamentally more complex. The first generation of plant metabolic engineering met with mixed success and produced unanticipated results — problems that are not necessarily restricted to manipulation of carotenogenesis. The reason is that predictive metabolic engineering relies on the establishment of both needed tools and an information infrastructure... [Pg.382]

Broun, P. and Somerville, C., Progress in plant metabolic engineering, Proc. Natl. Acad. Sci. USA 98, 8925, 2001. [Pg.386]

Sandmann, G., Rbmer, S., and Eraser, P.D., Understanding carotenoid metabolism as a necessity for genetic engineering of crop plants, Metabol. Eng. 8, 291, 2006. DellaPenna, D., Plant metabolic engineering. Plant Physiol. 125, 160, 2001. Wurtzel, E.T. and Grotewold, E., Plant metabolic engineering, in Encyclopedia of... [Pg.386]

Ralley, L. et ah. Metabolic engineering of ketocarotenoid formation in higher plants. [Pg.386]

Lee, P. and Schmidt-Dannert, C., Metabolic engineering towards biotechnological production of carotenoids in microorganisms, Appl. Microbiol. Biotechnol. V60, 1, 2002. [Pg.387]

Maury, J. et ah. Microbial isoprenoid production example of green chemistry through metabolic engineering, in Biotechnology for the Future, Springer-Verlag, Berlin,... [Pg.387]

Nessler, C.L., Metabolic engineering of plant secondary products. Transgenic Res. 3, 109, 1994. [Pg.387]

Ohlrogge, J., Plant metabolic engineering are we ready for phase two Curr. Opin. Plant Biol. 2, 121, 1999. [Pg.387]

Kholodenko, B.N. and Westerhoff, H.V., Metabolic Engineering in the Post Genomic Era, Horizon Bioscience, Norfolk, England, 2004. [Pg.387]

Gallagher, C.E., Cervantes-Cervantes, M., and Wurtzel, E.T., Surrogate biochemistry use of Escherichia coli to identify plant cDNAs that impact metabolic engineering of carotenoid accumulation, AppZ. Microbiol. Biotechnol. 60, 713, 2003. [Pg.390]

Mann, V. et al.. Metabolic engineering of astaxanthin production in tobacco flowers, Nat. Biotechnol. 18, 888, 2000. [Pg.394]


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