Metabolic engineering glycerol

Zhu, M. M. Lawman, P. D. Cameron, D. C. Improving 1,3-propanediol production from glycerol in a metabolically engineered E.coli by reducing accumulation of glycerol-3-phosphate, Biotechnol. Prog., 2002, 18, 694-699. 

Dharmadi, Y., Murarka, A. and Gonzalez, R. 2006. Anaerobic Fermentation of Glycerol by Escherichia Coli A New Platform for Metabolic Engineering. Biotechnol. Bioeng., 94, 821— 829. 

Boenigk R, Bowien S, Gottschalk G (1993) Fermentation of glycerol to PDO in continuous cultures of Citrobacter freundii. Appl Microbiol Biotechnol 38 453-457 Cameron DC, Altaras NE, Hoffman ML (1998) Metabolic engineering of propanediol pathways. Biotechnol Progr 14(116) 125... 

Menzel K, Zeng AP, Deckwer WD (1997) High concentration and productivity of PDO from cmtinuoiis fermentation of glycerol by Klebsiella pneumoniae. Enzyme Microbiol Technol 20(82) 86 Nakamura CE, Whited GM (2003) Metabolic engineering for the microbial production of... 

Gonzalez-Pajuelo M, Meynial-Salles I, Mendes F, Andrade JC, Vasconcelos I, Soucaille P. (2005b). Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol. Metab Eng, 1, 329-336. 

Dharmadi Y, Murarka A, Gonzalez R. (2006). Anaerobic fermentation of glycerol by Escherichia colt a new platform for metabolic engineering. Biotechnol Bioeng, 94(5), 821-829. 

In summary, metabolic engineering of E. coli led to a wide spectrum of succinate producer strains, several of which approached the theoretical limits of the succinate yield either under aerobic or anaerobic conditions from different carbon sources such as glucose or glycerol. An important feature of these successful studies was the... 

Blankschien MD, Clomburg JM, Gonzalez R. (2010). Metabolic engineering of Escherichia coli for the production of succinate from glycerol. Metab Eng, 12, 409-419. 

Zhang X, Shanmugam KT, Ingram LO. (2010). Fermentation of glycerol to succinate by metabolically engineered strains of Escherichia coli. Appl Environ Microbiol, 76, 2397-2401. 

Trinh C (2012) Elucidating and optimizing E. coli metaboUsms for obUgate anaerobic butanol and isobutanol production. Appl Microbiol Biotechnol 95(4) 1083-1094 Trinh CT, Srienc F (2009) Metabolic engineering of Escherichia coli for efficient conversion of glycerol to ethanol. Appl Environ Microbiol 75 6696-6705 Trinh CT, Carlson R, Wlaschin A, Srienc F (2006) Design, construction and performance of the most efficient biomass producing E. coli bacterium. Metab Eng 8 628-638 Trinh CT, Unrean P, Srienc F (2008) Minimal Escherichia coli ceU for the most efficient production of ethanol from hexoses and pentoses. Appl Environ Microbiol 74 3634-3643... 

PDO, and aldehyde dehydrogenase (AldD), the engineered S. blattae strains are able to produce 3-HP from glycerol, via the conversion of 1,3-PDO to 3-HPA and oxidation of 3-HPA to 3-HP. Using this metabolic engineering strategy. 

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