Synthesis of Oligosaccharides with Metabolically Engineered Cells

In summary, different strategies for the combinatorial biosynthesis of oHgosaccharides have been established successfully. However, some systems are Hmited mostly to bacterial glycosyltransferases because of their better expression in E. coli. The yeast systems seem to be better suited for expression of mammahan glycosyltransferases however, it remains to be demonstrated if they can be also utilized as living factories for the production of oligosaccharides. 

IgtB pl-4-galactosyltransferase from N. meningitides IgtA pi-3-glucosaminyltransferase from N. meningitides lacto-JV-neotetraose (17) [56] 

Le trisaccharide bound on a Gal motif (20), lacto-N-neoflicopentaose-1 (LNnF-1) (21) and 2 -flicosyllactose (22). 

8 (a) Saint-Jore-Dupas, C., Faye, L. and Gomord, V. (2007) Trends in Biotechnology, 25, 317-23. 

13 Liakatos, A. and Kunz, H. (2007) Current Opinion in Molecular Therapeutics, 9, 

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