Metabolic Engineering of Clostridium acetobutylicum for Butanol Fermentation

Metabolic Engineering of Clostridium acetobutylicum for Butanol Fermentation 

ABE fermentation using C. acetobutylicum faces several limitations (i) the fermentation product is a mixture of organic compounds with considerable differences in their market values (ii) -butanol, the most valuable product, also is the most toxic to the bacterial cells, which generally limited the n-butanol titer below 2% in a batch culture and (iii) ABE fermentation occurs only at a certain growth phase of C. acetobutylicum and usually stopped by the subsequent sporulation. 

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