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Strategies for metabolic engineering

The complete appreciation of cellular functions continues to lag behind the ability to analyse and manipulate the genome. As a result of this, strategies for metabolic engineering still remain largely reliant on successive rounds of genetic manipulation and analysis to hone the acquisition of the desired phenotype. Metabolic engineering [Pg.54]

Desai RP, Papoutsakis ET (1999) Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum. Appl Environ Microbiol 65 936—945... [Pg.128]

In this section, the strategies for metabolic and bioprocess engineering based on the omics technologies are described and their successful examples of applications are summarized. [Pg.2]

While strategies like metabolic engineering offer great promise for spawning new medicines, other areas of chemistry aim to clean up the Earth, improving people s health by ridding the air and waters of pollutants. [Pg.17]

To accomplish the many production schemes described in this chapter, genetic transformation of E. coli is necessary. Genetic transformation falls broadly into two categories, either increase of expression for a given polypeptide (overexpression) or decrease (knockdown or knockout), which are used to direct metabolite flow for metabolic engineering purposes. There are many effective strategies for each type of transformation. Bacterial transformation has been covered extensively elsewhere [205], so it is not covered at length here. [Pg.170]

Several strategies, including metabolic engineering, have been applied to improve the optical purity of LA optimizing enantioselective biosynthesis. For instance, a Lc. lactis mutant strain obtained by UV mutagenesis was able to produce d-LA from molasses and hydrolyzed sugarcane with 73% yield [308], while a recombinant strain of Lb. plantarum NCIMB 8826 produced... [Pg.432]

Bailey, J.E., Sburlati, A., Hatzimanikatis, V. et al. (2002) Inverse metabolic engineering a strategy for directed genetic engineering of useful phenotypes. Biotechnology and Bioengineering, 79, 568-579. [Pg.281]

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