Hydrogen production metabolic engineering

Stampfer W., Kosjek B., Moitzi C., Kroutil W. and Faber K. (2002) Biocatalytic asymmetric hydrogen transfer. Angew. Chem. Int. Ed., 41, 1014 Chartrain, M., Salmon, P.M., Robinson, D.K. and Buckland, B.C. (2000) Metabolic engineering and directed evolution for the production of pharmaceuticals. Curr. Opin. Biotechnol., 11, 209. 

METABOLIC ENGINEERING APPROACHES FOR THE IMPROVEMENT OF BACTERIAL HYDROGEN PRODUCTION BASED ON ESCHERICHIA COLI MIXED ACID FERMENTATION... 

In this review, we summarize the molecular biology of E.coli hydrogen production based on mixed acid fermentation, and our recent progress and approaches to enhance hydrogen production by metabolic engineering. 

Metabolic Engineering Approaches for the Improvement of Bacterial Hydrogen Production. .. 

Sode, K., Watanabe, M., Makimoto, H. and Tomiyama, M. (1998). Effect of hydrogenase 3 over-expression and disruption of nitrate reductase on fermentative hydrogen production in Escherichia coli, A metabolic engineering approach. Biohydrogen, O.R.Zaborsky Ed., Plenum Press 73-79. 

McNeely, K, Xu, Y., Bennette, N. et al (2010) Redirecting reductant flux into hydrogen production via metabolic engineering of fermentative carbon metabolism in a cyanobacterium. 

Rollin, J.A., Martin del Campo, J., Myung, S., Sun, F. et al. (2015) High-yield hydrogen production from biomass by in vitro metabolic engineering mixed sugars coutilization and kinetic modeling. 

Isopropanol is currently synthesized via three different methods indirect hydration of propylene (also called the sulfuric acid process), direct hydration of propylene, and catalytic hydrogenation of acetone. Efforts have been made to produce isopropanol by utilizing the TA76 strain of metabolically engineered E. coli. After the alcohol accumulates in the culture, production drastically decreases. Isopropanol removal by gas stripping allows for the continuation of the conversion process. Further development of this process may result in an alternative route to propylene by the dehydration of bioisopropanol. 

Enhancing hydrogen production through metabolic engineering... 

Kim, S., Seol, E., Oh, Y.-K., Wang, G.Y., Park, S., 2009. Hydrogen production and metabolic flux analysis of metabolically engineered Escherichia coli strains. International Journal of Hydrogen Energy 34, 7417—7427. 

Ryu, M.-H., Hull, N.C., Gomelsky, M., 2014. Metabolic engineering of Rhodobacter sphaeroides for improved hydrogen production. International Journal of Hydrogen Energy 39, 6384-6390. 

Wells, M.A., Mercer, J., Mott, R.A., Pereira-Medrano, A.G., Buija, A.M., Radianingtyas, H., Wright, P.C., 2011. Engineering a non-native hydrogen production pathway into Escherichia coli via a cyanobacterial [NiFe] hydrogenase. Metabolic Engineering 13, 445-453. 

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