Metabolic engineering 2,3-butanediols production

Bio-based Butanediols Production The Contributions of Cataiysis, Metabolic Engineering, and Synthetic Biology... 

Li ZJ, Jian J, Wei XX, Shen XW, Chen GQ. (2010c). Microbial production of meso-2,3-butanediol by metabolically engineered Escherichia coli under low oxygen condition. Appl Microbiol Biotechnol, 87, 2001-2009. 

Wang Q, Chen T, Zhao X, Chamu J. (2012b). Metabolic engineering of thermophilic Bacillus licheniformis for chiral pure D-2,3-butanediol production. Biotechnol Bioeng, 109, 1610-1621. 

Yim H, Haselbeck R, Niu W, Pujol-Baxley C, Burgard A, Boldt J, Khandurina J, Trawick JD, Osterhout RE, Stephen R, Estadilla J, Teisan S, Schreyer HB, Andrae S, Yang TH, Lee SY, Burk MJ, Van Dien S. (2011). Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol. Nat Chem Biol, 7, 445 52. 

This general strategy of using a feedback-resistant LPD mutant has been implemented by Genomatica to improve production of butanediol by . coli [131]. The specific mutation identified by Kim et al. was used to improve the production of -butanol, isobutanol, and pentanol by E. coli [132-134]. Thus, once again, metabolic engineering strategies that were first developed to improve ethanol production have been used to improve the production of other fuels and chemicals. 

Maddox IS (1996) Microbial production of 2,3-butanediol. In Rehm HJ, Reed G (eds) Biotechnology, vol 6,2nd edn. VCH, New York, pp 270-291 Madison LL, Huisman GW (1999) Metabolic engineering of poly(3-hydroxyalkanoates) from DNA to plastic. Microbiol Molec Biol Rev 63 21-53 Martin MC, Alonso JC, Suarez JE, Alvarez MA (2000) Generation of food-grade recombinant lactic add bacterium strains by site-specific recombination. Appl Environ Microbiol 66 2599-2604... 

It should be pointed out that a similar approach to the MMF technique, FBA-based technique such as OPTKNOCK also seeks to design engineered strains to couple growth and product formation and employ the metabolic pathway evolution strategy to improve the strain performance, e.g. development of an engineered and evolved E. coli strains for improved production of lactate (Fong et al. 2005), 1,4-butanediol (Yim et al. 2011), and various flavonoids (Fowler et al. 2009). 

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