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Tools for metabolic engineering

It is unlikely that it will be possible to make some measurements in batch fermentations, especially conunercial large-scale fermentations featuring the growth of yeast on a relatively uncharacterised medium such as wort. Batch cultures are inherently [Pg.51]

The transalptome can be monitored using a variety of techniques that allow the qitantiflcation of the degree of expression of entire genomes. Techniques such as serial analysis of gene expression (SAGE) and array analysis have been applied to yeast [Pg.52]

Control of the proteome is obviously of primary importance, since control at the cellular level is exerted via the activities of enzymes either via catalysis of individual steps in pathways, performing transport functions, or acting as regulatory proteins. The proteome is amenable to analysis via extractive techniques followed by chromatographic or electrophoretic separation and analysis, usually by mass spectrometry (MS) (Gavin et al, 2002 Picotti et al., 2013 Washburn, Worters, Yates, 2001). These methods have been particularly useful for gene annotation. [Pg.53]


Broun P. 2004. Transcription factors as tools for metabolic engineering in plants, Current Opinion Plant Biol 7 202-209. [Pg.40]

Wiechert, W. 2002. Modeling and simulation Tools for metabolic engineering. J Biotechnol 94 37-63. [Pg.224]

E. J. (2007) Construction and model-based analysis of a promoter library for E. coli an indispensable tool for metabolic engineering. BMC Biotech-noL, 7, 34. [Pg.579]

Wiechert W (2002) Modeling and simulation tools for metabolic engineering. J Biotechnol 94 37-63 Witholt B, Kessler B (1999) Perspectives of medium chain length poly(hydroxyalkanoates), a versatile set of bacterial bioplastics. CurrOpin Biotechnol 10 279-285... [Pg.83]

Keasling, J.D., 2012. Synthetic biology and the development of tools for metabolic engineering. Metab. Eng. 14, 189-195. [Pg.488]

Shiue, E., Prather, K.L., 2012. Synthetic biology devices as tools for metabolic engineering. Biochem. Eng. J. 65, 82-89. [Pg.494]

Further work at EniTecnologies was conducted with Rhodococcus strains. Rhodococ-cus was selected for its metabolical versatility, easy availability in soils and water, and remarkable solvent tolerance. Its capabilities for catalyzing diverse transformation reactions of crude oils, such as sulfur removal, alkanes and aromatics oxidation and catabolism caught their attention. Hence, genetic tools for the engineering of Rhodococcus strains have been applied to improve its biotransformation performance and its tolerance to certain common contaminants of the crude oil, such as cadmium. The development of active biomolecules led to the isolation and characterization of plasmid vectors and promoters. Strains have been constructed in which the careful over-expression of selected components of the desulfurization pathway leads to the enhancement of the sulfur removal activity in model systems. Rhodococcus, Gordona, and Nocardia were transformed in this way trying to improve their catalytic performance in BDS. In a... [Pg.283]

Fig. 1 The integration of systems biology tools with metabolic engineering for advancing industrial biotechnology is herein referred to as industrial systems biology. Industrial systems biology offers the opportunity to introduce new concepts in metabolic engineering and advances the development of cell factories through traditional fermentation for production of natural products and, after the advent of recombinant DNA technology, for the production of recombinant metabolites [1]... Fig. 1 The integration of systems biology tools with metabolic engineering for advancing industrial biotechnology is herein referred to as industrial systems biology. Industrial systems biology offers the opportunity to introduce new concepts in metabolic engineering and advances the development of cell factories through traditional fermentation for production of natural products and, after the advent of recombinant DNA technology, for the production of recombinant metabolites [1]...
Martinez-Garcia, E., Aparicio, X, de Lorenzo, V., and Nikel, P.l. (2014) New transposon tools tailored for metabolic engineering of gram-negative microbial cell factories. Front Bioeng. Biotechnol., 2, 46. [Pg.319]

Meynial-Salles I, Cervin MA, Soucaflle P (2005) New tool for metabolic pathway engineering in Escherichia coli one-step method to modulate expression of chromosomal genes. Appl Environ Microbiol 71 2140-2144... [Pg.200]


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Metabolic engineering

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