Metabolic engineering of glutamic acid production

This chapter reviews the molecular mechanism and metabolic engineering of glutamic acid production by C. glutamicum and potential use of glutamic acid as a building block for producing several other chemicals. 

Metabolic Engineering of Glutamic Acid Production by Corynebacterium Glutamicum... 

This chapter provides an overview of the establishment of glutamic acid fermentation and summarizes the molecular mechanism of glutamic acid production by C glutamicum. Moreover, it provides a discussion of metabolic engineering of C utamicum for glutamic acid production, as well as glutamic acid production by other microorganisms. 

Metabolic Engineering for Improvement of Glutamic Acid Production... 

Glutamic Acid Fermentation Discovery of Glutamic Acid-Producing Microorganisms, Analysis of the Production Mechanism, Metabolic Engineering, and Industrial Production Process... 

Vuoristo, K.S., Mars, A.E., Sangra, J.V., Springer, J. et al. (2015) Metabolic engineering of the mixed-acid fermentation pathway of Escherichia coli for anaerobic production of glutamate and itaconate. AAIB Express, 5, 61. 

Metabolic flux through the anaplerotic pathways (the intermediate pathways) will be limiting for amino acid production. Overexpression or deletion of the anaplerotic enzymes may help direct the flux toward increased precursor supply for better or overproduction of the amino acid. In branching amino acid biosynthetic pathways, weakening the competing branches to direct the available precursors and net carbon flux toward the overproduction of amino acids. The pyruvate node of the TCA cycle has been engineered for increased precursor supply for lysine, glutamate. 

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