Plant metabolic engineering whole plants

There has been much work conducted recently in the area of plant cell culture, or phytoproduction, especially where the product is a plant-unique mixture of Individual flavor substances such as vanilla extract of which vanillin is the major component. As well, the possibility of genetically engineering improved varieties of plants for high yield and consistent quality products is of considerable interest especially for more complex plant-unique flavors. Many flavor compounds are secondary metabolites for which a detailed understanding of their production is not well understood. Presently more knowledge exists In microbial metabolism relative to plant biosynthetic pathways and therefore has resulted in more successful development of microbial-based flavor bioprocessing. As well, scale-up of microbial cultures and isolated enzymes has become relatively common practice while the translation of plant cell culture to large commercial scales is not yet well established. This review will focus on the microbial whole cell and isolated enzyme systems for flavor production. 

Pasquali G, Porto DD, Fett-Neto AG. Metabolic engineering of cell cultures versus whole plant complexity in production of... 

Pasquali G, Porto DD, Fett-Neto AG (2006) Metabolic engineering of cell cultures versus whole plant complexity in production of bioactive monoterpene indole alkaloids recait progress related to old dilemma. J Biosci Bioeng 101 287-296. doi 10.1263/jbb.l0L287... 

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