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Metabolic engineering construction

Steinbuchel, A. and Lutke-Eversloh, T. 2003. Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochemical Engineering Journal 16 81-96. [Pg.39]

Kaup, B., Bringer-Meyer, S. and Sahm, H. (2004) Metabolic engineering of Escherichia coir, construction of an efficient biocatalyst for D-mannitol formation in a whole-cell biotransformation. Applied Microbiology and Biotechnology, 64 (3), 333-339. [Pg.163]

Aarnikunnas et aL (2002) used metabolic engineering of L. fermentum for the production of mannitol and pure L-lactic acid or pyruvate. The authors first developed genetic tools to modify L. fermentum and then proceeded to inactivate first IdhD gene and then IdhL gene to create a bacterium that could produce mannitol and either pure L-lactic acid or pyruvic acid in a single process. In bioreactor cultivations, the single mutant strain constructed by... [Pg.399]

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