Metabolic engineering Lactococcus lactis

Kleerebezemab, M., Hols, P., and Hugenholtz, J. 2000. Lactic acid bacteria as a cell factory rerouting of carbon metabolism in Lactococcus lactis by metabolic engineering. Enz. Microbial Technol., 26, 840-848. 

Genome-scale metabolic models Lactococcus lactis IL1403 First LAB genome-scale model metabolic engineering of central metabolism for diacetyl production Oliveira et al. (2005)... 

Mazumdar S, Blankschien M, Clomburg J, Gonzalez R (2013) Efficient synthesis of L-LA from glycerol by metabolically engineered Escherichia coli. Microb Cell Fact 12(7) 1-11 Melchiorsen CR, Jensen NBS, Christensen B, Jokumsen KV, Villadsen J (2001) Dynamics of pyruvate metabolism in Lactococcus lactis. Biotechnol Bioeng 74(4) 271-279. doi 10.1002/ bit. 1117... 

In addition, cofactor engineering has been used to deliberately modify the intracellular NADH/NAD+ ratio that plays a predominant role in controlling the Lactococcus lactis fermentation pattern. The introduction of the nox gene, which codes for a NADH oxidase (NOX) that converts molecular oxygen to water at the expense of NADH, to a strain with an inactivated copy of the aldB gene for a-acetolactate decarboxylase led to the efficient metabolism of the na-... 

Bongers, R., Hoefnagel, M. H. N., and Kleerebezem, M. 2005. High-level acetaldehyde production in Lactococcus lactis by metabolic engineering. Appl Environ. Microbiol., 71,1109-1113. 

Wisselink, H. W., Mars, A. E., van der Meer, P., Eggink, G, and Hugenholtz, J. 2004. Metabolic engineering of mannitol production in Lactococcus lactis influence of overexpression of mannitol-1-phosphate dehydrogenase in different genetic backgrounds. App. Environ. Microbiol., 76,4286 1292. 

Sybesma, W., Starrenburg, M., Kleerebezem, M., Mierau, I. et al (2003) Increased production of folate by metabolic engineering of Lactococcus lactis. Appl. Environ. Microbiol, 69, 3069-3076. 

Metabolic engineering strategies Increasing diacetyl production in Lactococcus lactis... 

Hols, R., Kleerebezem, M., Schanck, A. N., Ferain, T., Hugenholtz, J., Delcour, J., et al. (1999). Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering. Nature Biotechnology, 17,588-592. 

Oddone, G. M., Mills, D. A., Block, D. E. (2009). A dynamic, genome-scale flux model of Lactococcus lactis to increase specific recombinant protein expression. Metabolic Engineering, 11, 367-381. doi 10.1016/j.ymben.2009.07.007. 

Sybesma, W., Burgess, C., Starrenburg, M., Van Sinderen, D., Hugenholtz, J. (2004). Multivitamin production in Lactococcus lactis using metabohc engineering. Metabolic Engineering, 6, 109-115. 

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