Clostridium metabolic engineering

Desai RP, Papoutsakis ET (1999) Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum. Appl Environ Microbiol 65 936—945... 

Nakayama S, Kosaka T, Hirakawa H, Matsuura K, Yoshino S, Furukawa K (2008) Metabolic engineering for solvent productivity by downregulation of the hydrogenase gene cluster hupCBA in Clostridium saccharoperbutylacetonicum strain N1 -4. Appl Microbiol Biotechnol 78 483 493... 

Jang, Y.-S., Im, JA., Choi, S.Y., Lee, J.I. et al (2014) Metabolic engineering of Clostridium acetobutylicum for butyric acid production with high butyric acid selectivity. Metab. Eng., 23, 165-174. 

Dusseaux, S., Croux, C., Soucaille, P., and Meynial-Salles, I. (2013) Metabolic engineering of Clostridium acetobutylicum ATCC 824 for the high-yield production of a biofuel composed of an isopropanol/butanol/ethanol mixture. Metab. Eng., 18, 1-8. 

Mermelstein, L.D., Papoutsakis, E.T., Petersen, D.J., and Bennett, G.N. (1993) Metabolic engineering of Clostridium acetobutylicum ATCC 824 for increased solvent production by enhancement of acetone formation enzyme activities using a synthetic acetone operon. Biotechnol. Bioeng., 42, 1053—1060. Qureshi, N., Li, X.-L., Hughes, S, Saha, B.C. et al. (2006) Butanol production from com fiber xylan using Clostridium acetobutylicum. Biotechnol. Progr, 22, 673 -680. 

Lee J, Jang Y-S, Choi SJ, Im JA, Song H, Cho JH, Seung DY, Papoutsakis ET, Bennett GN, Lee SY. (2012). Metabolic engineering of Clostridium acetobutylicum ATCC 824 for isopropanol-butanol-ethanol fermentation. Appl Environ Microbiol, 78,1416-1423. 

Lutke-Eversloh T, Bahl H. (2011). Metabolic engineering of Clostridium acetobutylicum recent advances to improve butanol production. Curr Opin Biotechnol, 22, 634—647. 

Sillers R, Chow A, Tracy B, Papoutsakis ET. (2008). Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance. Metab Eng, 10,321—332. 

Gonzalez-Pajuelo M, Meynial-Salles I, Mendes F, Andrade JC, Vasconcelos I, Soucaille P. (2005b). Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol. Metab Eng, 1, 329-336. 

Guedon, E., Desvaux, M., and Petitdemange, H. (2002) Improvement of cellulolytic properties of Clostridium cellidolyticum by metabolic engineering. Appl. Environ. Microbiol, 68,... 

Higashide, W., Li, Y., Yang, Y, and Liao, J.C. (2011) Metabolic engineering of Clostridium cellulolyticum for production of isobutanol from cellulose. Appl EnvirorL Microbiol., 77, 2727—2733. 

Metabolic Engineering of Clostridium acetobutylicum for Butanol Fermentation... 

Liitke-Eversloh, T. (2014) Application of new metabolic engineering tools for Clostridium acetobutylicum. Appl. Microbiol Biotechnol, 98, 5823-5837. 

Zhang, Y. and Yang, S.-T. (2009) Metabolic engineering of Clostridium tyrobutyricum for production of biofuels and bio-based chemicals, 3375890, 234. 

Mermelstein LD, Papoutsakis ET, Petersen DJ, Bennett GN (1993) Metabolic engineering of Clostridium acetobutylicum ATCC 824 for increased solvent production by enhancement of acetone formation enzyme activities using a synthetic acetone operon. Biotechnol Bioengin 42 1053-1060... 

Papoutsakis ET, Bennett GN (1999) Molecular regulation and metabolic engineering of solvent production by Clostridium acetobutylicum. In Papoutsakis ET, Lee SY (eds) Bioprocess technology, vol 24. Marcel Dekker, New York,... 

Lee JY et al (2009) Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production. Biotechnol J 4(10) 1432-40... 

For acetone-butanol-ethanol (ABE) fermentation, the broth contains about 25-35 g/1 of mixed solvents. Butanol concentration is usually less than 20 g/1, which makes its recovery by distillation expensive. This low butanol concentration in the fermentation broth is related to the inability of Clostridium species to produce more butanol due to solvent toxicity (Lee et al., 2008). Metabolic engineering and advanced fermentation techniques are ongoing to enhance the organisms abilities to produce and tolerate higher concentrations of butanol and increase productivity. Several integrated fermentation and recovery processes, such as fed-batch fermentation with pervaporation and continuous fermentation with gas stripping, have been reviewed elsewhere (Lee et al., 2008). 

Cooksley, CM., Zhang, Y, Wang, H., Redl, S., Winzer, K., Minton, N.R, 2012. Targeted mutagenesis of the Clostridium acetobutylicum acetone-butanol-ethanol fermentation pathway. Metabolic Engineering 14 (6), 630-641. 

Besides S. cerevisiae, Zymomonas mobilis and C. acetobutylicum have been also engineered with metabolic pathway for xylose and/or arabinose utilization. Clostridium... 

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