Metabolic engineering Saccharomyces cerevisiae

Hasunuma T, Sung KM, Sanda T, Yoshimura K, Matsuda F, Kondo A. (2011b). Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae. Appl Microbiol Biotechnol, 90, 997-1004. 

Sauer, M., Branduardi, R, Valli, M., etal. (2004). Productionof L-ascorbic Acid by Metabolically Engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii, Appl. Environ. Microbiol., 70, pp. 6086-6091. 

J. et al. (2014) Production of beta-carotene by metabolically engineered Saccharomyces cerevisiae. Chin J biotech, 30, 1204-1216. 

Costenoble, R., Adler, L., Niklasson, C., and Liden, G. 2003. Engineering of the metabolism of Saccharomyces cerevisiae for anaerobic production of mannitol. FEMS Yeast Res., 3,17-25. 

Porro D, Brambilla L, Ranzi BM, Martegani E, Alberghina L. (1995). Development of metabol-ically engineered Saccharomyces cerevisiae cells for the production of lactic acid. Biotechnol Prog, 11, 294-298. 

Because most natural carotenoids are present at very low abundance and are difficult to purify, metabolic engineering provides a powerful alternative, and various carotenoids, such as lycopene, /3-carotene, canthaxanthin, zeaxanthin, torulene, neurosporaxanthin, and astaxanthin, have been successfully synthesized in non-carotenogenic microbes, such as E. coli, Saccharomyces cerevisiae, and Neurospora crassa. This research is summarized in several of reviews [65,108,110]. 

Mutka, S.C., Bondi, S.M., Carney, J.R. et al. (2006) Metabolic pathway engineering for complex polyketide biosynthesis in Saccharomyces cerevisiae. FEMS Yeast Research, 6, 4047. 

KlapaMI, Park SM, Sinskey AJ, Stephanopoulos G (1999) Biotechnol Bioeng 62 375 Lirxenburger H, Wittmann C, Heinzle E (1998) Metabolic flux studies in Saccharomyces cerevisiae using dynamic membrane mass spectrometry. 2" Conference on Metabolic Engineering, Elman, Germany. 

Kuyper, M., Hartog, M. M., Toirkens, M. J., Almering, M. J., Winkler, A. A., van Dijken, J. P., and Pronk, J. T., Metabolic engineering of a xylose-isomerase-expressing Saccharomyces cerevisiae strain for rapid anaerobic xylose fermentation. FEMS Yeast Res 2005, 5 (4-5), 399-409. 

Fig. 3 Metabolic engineering for L-lactic acid production in Saccharomyces cerevisiae. The abbreviation of enzymes is described as follows LDH bovine lactate dehydrogenase, PDC pyruvate decarboxylase complex encoded by the genes PDC1, PDC5, and PDC6, which were deleted in this strategy...

Nielsen J, Jewett MC (2006) Impact of systems biology on metabolic engineering of Saccharomyces cerevisiae. In 25th International specialized symposium on Yeasts (ISSY25), Blackwell, Espoo, Finland... 

Becker, J. V., Armstrong, G. O., van der Merwe, M. J., Lambrechts, M. G., Vivier, M. A., Pretoiius, 1. S. (2003) Metabolic engineering of Saccharomyces cerevisiae for the synthesis of the wine-related antioxidant resveratrol. FFMS Yeast Research, 4, 79-85. 

Shiba Y, Paradisea EM, Kirbya J, Ro D-K Keasling JD. Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae for high-level production of isoprenoids. Metabol. Eng. 2007 9 160-168. 

Over the past 30 years, a few researchers have reported the presence of xylose isomerase in a number of yeasts and fungi capable of rapid xylose metabohsm. Because of difficulties in using genetically engineered Saccharomyces, Freer et al. [38] re-examined Rhodosporidium toruloides to see if they could confirm an earlier report that this yeast produces xylose isomerase. They reasoned that the heterologous expression of an eukaryotic enzyme could fadfitate genetic engineering of xylose metabolism in S. cerevisiae. Unfortunately, they found that R. toruloides uses an oxidoreductase system fike other eukaryotes. Other approaches, however, have been more successful. 

Steinle, A., Bergander, K., and Steinbiichel, A. (2009) Metabolic engineering of Saccharomyces cerevisiae for production of novel cyanophydns with an extended range of constituent amino acids. Appl. Environ. Microbiol., 75, 3437-3446. 

Simon Ostergaard, Lisbeth Olsson, Jens Nielsen, Metabolic Engineering of Saccharomyces cerevisiae. Microbiology and Molecular Biology Reviews, 64 (2000), 34-50. 

Metabolic Engineering of Saccharomyces cerevisiae for Xylose Utilization... 

Keywords. Xylose, Saccharomyces cerevisiae. Ethanol, Lignocellulose, Metabolic engineering... 

---