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Improving Cellular Performance by Genetic and Metabolic Engineering

Improving Cellular Performance by Genetic and Metabolic Engineering [Pg.662]

O Callaghan et al. [140]. Their analysis of seven CHO producer cell lines based on data-driven, in-depth protein formation models revealed that sufficient light chain (and also heavy chain) mRNA was produced but downstream protein formation was hampered for individual reasons in the cells. [Pg.663]

the interplay of miRNA levels with protein formation surely represents a highly attractive research field for improving cellular performance, at the same time asking for basic research to unravel underlying regulatory mechanisms. [Pg.663]

As outlined earlier, by-products such as lactate can inhibit cell-specific productivity as well. Consequently, multiple efforts were made to reduce lactate production either by increasing the drain from the precursor pyruvate [144,145] or by reducing Idh activity [146,147]. [Pg.663]

Besides, Tabuchi and Sugiyama [65] recently achieved maximum cell-specific mAB productivities greater than 100 pgcell day with a DHFR-deficient CHO DUK-XBll cell line overexpressing the taurine transporter TAUT and the alanine aminotransferase 1 ALTl for amplifying the internal pyruvate and glutamate supply. [Pg.663]




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Cellular engineering

Cellular metabolism

Genetic Engineering and

Genetic engineering

Genetically engineered

Genetics genetic engineering

Improving performance

Metabolic engineering

Metabolism genetics and

Performance improvement

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