Improving Cellular Performance by Genetic and Metabolic Engineering

Improving Cellular Performance by Genetic and Metabolic Engineering 

O Callaghan et al. [140]. Their analysis of seven CHO producer cell lines based on data-driven, in-depth protein formation models revealed that sufficient light chain (and also heavy chain) mRNA was produced but downstream protein formation was hampered for individual reasons in the cells. 

the interplay of miRNA levels with protein formation surely represents a highly attractive research field for improving cellular performance, at the same time asking for basic research to unravel underlying regulatory mechanisms. 

As outlined earlier, by-products such as lactate can inhibit cell-specific productivity as well. Consequently, multiple efforts were made to reduce lactate production either by increasing the drain from the precursor pyruvate [144,145] or by reducing Idh activity [146,147]. 

Besides, Tabuchi and Sugiyama [65] recently achieved maximum cell-specific mAB productivities greater than 100 pgcell day with a DHFR-deficient CHO DUK-XBll cell line overexpressing the taurine transporter TAUT and the alanine aminotransferase 1 ALTl for amplifying the internal pyruvate and glutamate supply. 

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