Systems biology and metabolic engineering in LAB

1 Kinetic modeis and genome-scaie metabolic models 

Kinetic models capture the dynamics of the specific pathways and, in particular, interactions at the enzyme-substrate-product level that are translated into intracellular fluxes and transport dynamics. Thus a series of pathway modifications can be implemented, ranging from enzyme inactivation or enzyme adjunction to modification of 

Kinetic models Lactococcus lactis IL1403 First model of glycolysis in LAB Hoefnagel, Starrenburg, et al. (2002) and Hoefnagel, Van Der Burgt, et al. (2002) 

Genome-scale metabolic models Lactococcus lactis IL1403 First LAB genome-scale model metabohc engineering of central metabolism for diacetyl production Oliveira et al. (2005) 

Finally, three strains of Lactobacillus reuteri have been used for GEMM reconstruction. Lactobacillus reuteri JCMl 12 had been used for medium optimization to enhance vitamin B12 biosynthesis by insertion of the biosynthetic pathway from Lactobacillus plantarum WCFSl (Santos et al., 2008). Furthermore, two isolates derived from breast milk have been used for metabolic model reconstructions (Saulnier et al., 2011). Lactobacillus reuteri strain 55730 has been identified as an important probiotic strain, and hence efforts were made to identify its functional properties via the integration of omics data, in particular, the amino acid biosynthesis capacity and vitamin production were detailed thanks to the model and proposed as part of traits enhancing probiotic effects. Such study represents an important step toward a metagenome-scale model and its application in industrial fermentations (Branco Dos Santos, De Vos, Teusink, 2013). 

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