Syrian hamster embryo cell

Syrian hamster embryo cells (clonal assay) Cell transformation No data — Amacher and Zelljadt 1983... 

Amacher DE, Zelljadt I. 1983. The morphological transformation of Syrian hamster embryo cells by chemicals reportedly nonmutagenic to Salmonella typhimurium. Carcinogenesis 4 291-295. 

Hayashi, N. Hasegawa, K. Barrett, J. C. Tsutsui, T. Estrogen-induced cell transformation and DNA adduct formation in cultured Syrian hamster embryo cells. Mol. Carcinog. 1996, 16, 149-156. 

Chinese hamster ovary cells Syrian hamster embryo cells Chromosomal aberration, DNA repair, mitotic disturbance NA + Bauchingerand Schmid 1972 Costa et al. 1982 Robison et al. 1984 Zelikoff et al. 1988 Ariza et al. 1998... 

Dunkel VC, Pienta RJ, Sivak A, et al. 1981. Comparative neoplastic transformation responses of Balb/3T-3 cells, Syrian hamster embryo cells, and Rauscher murine leukemia virus-infected Fischer 344 rat embryo cells to chemical carcinogens. J Nat Cancer Inst 67 1303-1315. 

Pienta RJ, Poiley JA, Lebherz WB III. 1977. Morphological transformation of early-passage golden Syrian hamster embryo cells derived from cryopreserved primary cultures as a reliable in vitro bioassay for identifying diverse carcinogens. Int J Cancer 19 642-655. 

Hepatocyte primary cultures Hepatocyte primary cultures Cell transformation Syrian hamster embryo cells... 

Aniline transformed the Balb/3T3 mouse cell line at doses of 0.8 to 100 /dose-response effect), but not the Syrian hamster embryo cells (Dunkel et al. 1981). Results were negative in DNA damage assays in Escherichia coli (Mamber et al. 1983) and Bacillus subtilis (McCarroll et al. 1981). 

Lee, T.C., M. Oshimura, and J.C. Barrett. 1985. Comparison of arsenic-induced cell transformation, cytotoxicity, mutation and cytogenetic effects in Syrian hamster embryo cells in culture. Carcinogenesis 6 1421-1426. 

On the other hand, mercuric chloride, at a concentration of 50 /iM, also has been shown to enhance viral transformation of Syrian hamster embryo cells [252],... 

Pienta RJ. 1980. Evaluation and relevance of the Syrian hamster embryo cell system. Applied Methods in Oncology 3 149-169. 

Tsutsui T, Hayashi N, Maizumi H, et al. 1997. Benzene-, catechol-, hydroquinone- and phenol-induced cell transformation, gene mutations, chromosome aberrations, aneuploidy, sister chromatid exchanges and unscheduled DNA synthesis in Syrian hamster embryo cells. Mutat Res 373 113-123. 

In Vitro Syrian Hamster Embryo Cell Transformation Test. 308... 

Isfort, R.J., Cody, D.B., Doerson, C., Kerckaert, G.A., and LeBoeuf, R.A., Alterations in cellular differentiation, mitogenesis, cytoskeleton and growth characteristics during Syrian hamster embryo cell multistep in vitro transformation, Int. J. Cancer, 59, 114, 1994. 

LeBoeuf, R.A., and Kerckaert, G., Enhanced morphological transformation of early passage Syrian hamster embryo cells cultured in medium with a reduced bicarbonate concentration and pH, Carcinogenesis, 8, 680, 1987. 

Syrian hamster embryo cells Enhanced transformation by SA7 adenovi res No data - Hatch et al. 1983... 

Hatch GG, Mamay PD, Ayer ML, et al. 1983. Chemical enhancement of viral transformation in Syrian hamster embryo cells by gaseous and volatile chlorinated methanes and ethanes. Cancer Res 43 1945-1950. 

Farmer P Committee on Mutagenicity of Chemicals in Food, Consumer Products and the Environment ILSl/HESl research programme on alternative cancer models results of Syrian hamster embryo cell transformation assay. International Life Sciences Institute/Health and Environmental Science Institute. Toxicol Pathol 2002 30 536-8. 

Acetamide was mutagenic in Escherichia coli and Salmonella typhimurium-, this effect was independent of dose. Acetamide produced morphological transformation in Syrian hamster embryo cells in the absence of metabolic activation. However, acetamide did not induce reversions in several Salmonella typhimurium strains. ... 

Amitrole was not genotoxic in bacterial assays and cultured mammalian cells or in rodents exposed in vivo it did induce transformation of Syrian hamster embryo cells in vitro)... 

Inhibition of gap-junetional intercellular communieation, Chinese hamster V79 eells in vitro Inhibition of gap-junctional intercellular communication, Chinese hamster V79 cells in vitro Inhibition of gap-junctional intercellular communication, Syrian hamster embryo cells in vitro Inhibition of gap-junctional intercellular communication, Chinese hamster V79 cells in vitro Inhibition of gap-junctional intercellular communication, Chinese hamster V79 cells and Syrian hamster embryo cells in vitro... 

In one of two studies, di(2-ethylhexyl) phthalate induced a small increase in sister chromatid exchange frequencies in Chinese hamster ovary cells cultured without but not with exogenous metabolic activation. In other studies conducted only without metabolic activation, it caused no increase in sister chromatid exchanges in either Chinese hamster Don cells or rat liver RL4 cells. Di(2-ethylhexyl) phthalate did not induce micronuclei in Chinese hamster ovary cells or in cultured rat hepatocytes, whereas the induction of micronuclei by di(2-ethylhexyl) phthalate in Syrian hamster embryo cells has been reported. 

Chromosomal aberrations were not induced by di(2-ethylhexyl) phthalate in any of eight studies in various types of cultured cells in the absence of metabolic activation. Only three of these studies for chromosomal aberrations included an exogenous metabolic activation system. Of these, one, using Syrian hamster embryo cells, found an increase in aberration frequency. Weak effects were detected for the induction of aneuploidy and mitotic division aberrations in Chinese hamster lung cells. 

RLV/Fischer rat assay without the addition of an exogenous metabolic activation system. In a single study, mouse JB6 epidermal cells were transformed by di(2-ethyl-hexyl) phthalate without activation and in one of two studies a weak response was reported in the CSHIOT A cell transformation assay with di(2-ethylhexyl) phthalate in either the absence or presence of exogenous metabolic activation. BALB/c-3T3 cells were not transformed by di(2-ethylhexyl) phthalate with or without metabolic activation. Di(2-ethylhexyl) phthalate inhibited gap-junctional intercellular communication in Chinese hamster V79 cells in six of seven studies, but not in one study of liver cells of cynomolgus monkeys in vivo. Di(2-ethylhexyl) phthalate treatment of Syrian hamster embryo cells in a two-stage exposure with 12-O-tetradecanoylphorbol 13-acetate resulted in superinduction of ornithine decarboxylase, an early event in morphological transformation no effect was seen after a one-stage treatment with di(2-ethylhexyl) phthalate alone. 

Unscheduled DNA synthesis was not induced in either mouse or hirman primary hepatocyte cultures with mono(2-ethylhexyl) phthalate, and neither this metabolite nor 2-ethylhexanol induced mutations in mouse lymphoma cells in vitro. Mono(2-ethyl-hexyl) phthalate induced sister-chromatid exchange in Chinese hamster V79 cells and chromosomal aberrations in Syrian hamster embryo cells. It also induced transformation in Syrian hamster embryo cells, but not in mouse C3H10T /2 cells. Gap-junctional intercellular communication was inhibited by mono(2-ethylhexyl) phthalate in Syrian hamster embryo cells and in Chinese hamster V79 cells. As reported in an abstract (Baker et al., 1996), this function was also inhibited in rat and mouse hepatocytes, but not in Syrian hamster or human hepatocytes. 

Barrett, J.C. Lamb, P.W. (1985) Tests with the Syrian hamster embryo cell transformation... 

Mettang, T, Thomas, S., Kiefer, T, Fischer, F.-R, Kuhlmann, U., Wodarz, R. Rettenmeier, A.W. (1996b) Uraemic praritus and exposure to di(2-ethylhexyl) phthalate (DEHP) in haemodialysis patients. Nephrol. Dial. Transplant., 11, 2439-2443 Mikalsen, S.-O. Saimer, T. (1993) Intercellular communication in colonies of Syrian hamster embryo cells and the susceptibility for morphological transformation. Carcinogenesis, 14, 251-257... 

Mikalsen, S., Holen, I. Sarmer, T. (1990) Morphological transformation and catalase activity of Syrian hamster embryo cells treated with hepatic peroxisome proliferators, TPA and nickel sulphate. Cell Biol. Toxicol., 6, 1-13... 

Cell transformation, Syrian hamster embryo cells in vitro NT 50 pg/mL Tu et al. (1986)... 

Tu, A., Hallowell, W., Pallotta, S., Sivak, A., Lubet, R.A., Curren, R.D., Avery, M.D., Jones, C., Sedita, B.A., Huberman, E., Tennant, R., Spalding, J. Kouri, R E. (1986) An interlaboratory comparison of transformation in Syrian hamster embryo cells with model and coded chemicals. Environ. Mutag., 8, 77-98... 

Ethylbenzene was non-mutagenic in bacteria, yeast and insects. In mammalian cells, it was inactive in inducing sister chromatid exchanges in Chinese hamster embryo cells but very weakly positive in cultured human l5miphocytes. It did not induce micronuclei in vivo, although it was positive in Syrian hamster embryo cells in vitro. It also caused cell transformation in these cells. Ethylbenzene induced mutations in the mouse lymphoma assay, but only at the highest non-lethal concentration tested. 

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