Hepatocyte primary culture DNA repair test

Williams GM, Tong C, Brat SV. 1985. Tests with the rat hepatocyte primary culture/DNA-repair test. In Ashby J, de Serres FJ, et al., eds. Progress in mutation research. Vol. 5. Evaluation of short-term tests for carcinogens. Amsterdam, The Netherlands Elsevier Science Publishers,... 

McQueen, C.A. and Williams, G.M. (1981). Characterization of DNA repair elicited by carcinogens and drugs in the hepatocyte primary culture/DNA repair test. J. Toxicol. Environ. Health 8 463-477. 

Williams GM, Laspia MF, Dunkel VC. 1982. Reliability of the hepatocyte primary culture/DNA repair test in testing of coded carcinogens and noncarcinogens. Mutat Res 97 359-370. 

Williams, G.M., Tong, C. Ved Brat, S. (1985) Tests with the rat hepatocyte primary culture/ DNA repair test. Prog. Mutat. Res., 5, 341-345... 

WHO (1993) Guidelines for Drinking Water Quality, 2nd Ed., Vol. 1, Recommendations. Geneva Williams, G.M., Laspia, M.F. Dunkel. VC. (1982) Reliability of the hepatocyte primary culture/DNA repair test in testing of coded carcinogens and noncarcinogens. Mutat. Res., 97,... 

Mori, H., Sugie, S., Yoshimi, N., Iwata, H.,Nishikawa, A., Matsukubo, K.. Shimizu. H. Hirono, I. (1988) Genotoxicity of a variety of hydrazine derivatives in the hepatocyte primary culture/ DNA repair test using rat and mouse hepatocytes. Jpn. J. Cancer Res., I ). 204-211... 

By using the hepatocyte primary culture DNA repair test [247] with liver cells of adult ACI rats, an essay that is capable to discern true carcinogens from solely mutagenic compounds such as quercetin and other bioactive flavonoids, Japanese workers [248] were able to show that ptaquiloside is a genotoxic carcinogen. The key evidence was that ptaquiloside induced DNA repair in the exposed liver cells, which could be interpreted as an intrusion into the DNA molecule that caused enough distortions to trigger the reconstruction mechanism. 

McQueen CA, Williams GM. 1987. The hepatocyte primary culture/DNA repair test using hepatocytes from several species. Cell Biology and Toxicology 3(2) 209-218. 

Mori H, Yoshimi N, Sugie S, et al. 1988. Genotoxicity of epoxy resin hardeners in the hepatocyte primary culture/DNA repair test. Mutat Res 204(4) 683-688. 

Mutagenicity assays using whole cell systems may be more sensitive than the S. typhimurium assay and could possibly be less prone to the difficulties associated with the use of relatively high substrate concentrations. For example, NNN is mutagenic toward S. typhimurium only at very high concentrations, whereas it is readily detected in the hepatocyte primary culture-DNA repair test... 

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