Cell transformation assays

C3T3 mouse cells (BALE cell transformation assay) Cell transformation No data (+) Tuetal. 1985... [Pg.163]

The second example focuses on qualitative evaluation of cancerogenic potential of some perfluorinated compounds using both QSARs models and an in vitro cell transformation assay. [Pg.172]

Fig. 5 BALB/c 3T3 cell transformation assay example of focus induced in BALB/c 3T3 clone A31-1-1 after exposure to a carcinogenic compound. Cells are stained with Giemsa stain...

Fig. 6 BALB/c 3T3 cell transformation assay Colony formation assay...

Fig. 9 BALB/c 3T3 cell transformation assay transformation frequency of PFOA and PFNA. Red bar is the control group (untreated cells)...

ECVAM (2010) Balb/c 3T33 Cell Transformation Assay - Prevalidation Study Report... [Pg.205]

Vanparys P, Schechtman L, Aardema M, Hayashi M, Hoffmann S, Gribaldo L, Corvi R (2010) Balb/c 3T3 Cell Transformation Assay - Prevalidation Study Report. ECVAM Balb/c 3T3 CTA PRP report vl - 30 July 2010... [Pg.205]

Sanner T, Rivedal E. 1985. Tests with the Syrian hamster embryo (SHE) cell transformation assay. In Ashby J, de Serres FJ, et al., eds. Progress in mutation research. Vol. 5. Evaluation of short-term tests for carcinogens. Amsterdam, The Netherlands Elsevier Science Publishers, 665-671. Sato M, Ishizu S, Momotuni H. 1975. [Determination of acrylonitrile, cyanide and thiocyanate in blood and urine.] Jap J Ind Health 17 99-105. (Japanese). [Pg.118]

Water-soluble nickel salts enter the cells with relative ease but are less effective than crystalline particulates in the cell transformation assay, when using hamster cells [429, 430]. However, an enhancement of viral transformation of cells has been found [431, 432], In addition, a synergistic effect of cigarette smoke extracts, benzo[a]pyrene and nickel sulphate on the morphological transformation of hamster embryo cells has also been obtained [433], It was suggested that nickel salts are more potent as promoters than they are as initiators [434],... [Pg.219]

The SHE cell transformation assay at pH 6.7 is conducted to determine the ability of a test article to induce morphological transformation in cultured SHE cells. The SHE cell transformation assay is one of the most widely used cell transformation assays. The endpoints of this assay are related to the conversion of normal cells into preneoplastic or neoplastic cells. The assay provides a valuable tool in the process of assessment and evaluation of the carcinogenic potential of a test article.2 28... [Pg.308]

Engelhardt, G., Schwind, K.-R., and Mulller, B., The testing of chemicals in the Syrian hamster embryo (SHE) cell transformation assay for assessment of carcinogenic potential, Toxicology In Vitro, 8, 213, 2004. [Pg.313]

Custer L, Gibson DP, Aardema MJ, Le Boeuf RA. A refined protocol for conducting the low pH 6.7 Syrian hamster embryo (SHE) cell transformation assay. Mutat Res 2000 455 129-39. [Pg.142]

Farmer P Committee on Mutagenicity of Chemicals in Food, Consumer Products and the Environment ILSl/HESl research programme on alternative cancer models results of Syrian hamster embryo cell transformation assay. International Life Sciences Institute/Health and Environmental Science Institute. Toxicol Pathol 2002 30 536-8. [Pg.142]

In most instances the specimens will be self-evident (e.g., samples of blood, plasma, serum, urine, spinal fluid, aqueous humor, organs, tissues, and tissue fractions that are taken from a test system with the intention of performing an examination or analysis). In other instances the definition may not be as clear. For example, the assay plates used in the mammalian cell transformation assay and the mammalian point mutation assay are considered specimens even though they bear many of the attributes of a test system. For these assays, the originally plated cells plus media and excipients are the test system. After treatment with the test or... [Pg.46]

RLV/Fischer rat assay without the addition of an exogenous metabolic activation system. In a single study, mouse JB6 epidermal cells were transformed by di(2-ethyl-hexyl) phthalate without activation and in one of two studies a weak response was reported in the CSHIOT A cell transformation assay with di(2-ethylhexyl) phthalate in either the absence or presence of exogenous metabolic activation. BALB/c-3T3 cells were not transformed by di(2-ethylhexyl) phthalate with or without metabolic activation. Di(2-ethylhexyl) phthalate inhibited gap-junctional intercellular communication in Chinese hamster V79 cells in six of seven studies, but not in one study of liver cells of cynomolgus monkeys in vivo. Di(2-ethylhexyl) phthalate treatment of Syrian hamster embryo cells in a two-stage exposure with 12-O-tetradecanoylphorbol 13-acetate resulted in superinduction of ornithine decarboxylase, an early event in morphological transformation no effect was seen after a one-stage treatment with di(2-ethylhexyl) phthalate alone. [Pg.115]

Sanchez, J.H., Abemethy, D.J. Boreiko, C.J. (1987) Lack of di-(2-ethylhexyl) phthalate activity in the C3H/10T1/2 cell transformation system. Toxicol. In Vitro, 1, 49-53 Sarmer, T. Rivedal, E. (1985) Tests with the Syrian hamster embryo (SHE) cell transformation assay. Prog. Mu tat. Res., 5, 665-671 Sasakawa, S. Mitomi, Y. (1978) Di-2-ethylhexyl phthalate (DEHP) content of blood or blood components stored in plastic bags. Vox Sang., 34, 81-86 Saxena, D.K., Srivastava, S.P, Chandra, S.V. Seth, P.K. (1985) Testicrrlar effects of di-(2-ethylhexyl) phthalate (DEHP) histochemical and histopathological alteratiorrs. Ind. Health, 23, 191-198... [Pg.143]

Gibson, D.R, Brauninger, R., Shaffi, H.S., Kerckaert, GA., LeBoeuf, R.A., Isfort, R.J. Aardema, M.J. (1997) Induction of micronuclei in Syrian hamster embryo cells comparison to results in the SHE cell transformation assay for National Toxicology Program test chemicals. Mutat. Res., 392, 61-70... [Pg.261]

Kerckaert, G.A., LeBoeuf, R.A. Isfort, R.J. (1998) Assessing the predictiveness of the Syrian hamster embryo cell transformation assay for determining the rodent carcinogenic potential of single ring aromatic/nitroaromatic amine compounds. Toxicol. Sci., 41, 189-197... [Pg.311]

Sanner, T. Rivedal, E. (1985) Tests with the Syrian hamster embryo (SHE) cell transformation assay. In Ashby, J., de Serres, F.J., Draper, M., Ishidate, M., Jr, Margolin, B.H., Matter, B.E. Shelby, M.D., eds, Progress in Mutation Research, blume 5, Evaluation of Short-Term Tests for Carcinogens. Report of the International Programme on Chemical Safety s Collaborative Study on in vitro assays, Amsterdam, Elsevier Science, pp. 665-671 Savela, A., Vuorela, R. Kauppinen, T. (1999) ASA 1997 [Reviews 140], Helsinki, Finnish Institute of Occupational Health, pp. 47-48 (in Finnish)... [Pg.318]

Nitromethane gave negative results in all short-term tests for genetic effects, with the exception of a cell transformation assay in which it was positive at high concentration. [Pg.498]

LeBoeuf RA, Kerchaert GA, Aardema MJ, et al. 1996. The pH 6.7 Syrian hamster embryo cell transformation assay for assessing the carcinogenic potential of chemicals. Mutat Res 356 85-127. [Pg.437]

Barrett, J. C. Lamb, P. W. (1985) Tests with the Syrian hamster embryo cell transformation assay. Prog. Mutat. Res., 5, 623-628... [Pg.93]

Hatch. GG. Mamay, P.D., Ayer, M.L.. Casto, B.C. Nesnow, S. (1982) Methods for detecting gaseous and volatile carcinogens using cell transformation assays. Environ. Sci. Res., 25,75-90... [Pg.304]

Most of the broad of tests for genotoxic activity have produced negative results, but positive results were obtained in a cell transformation assay and conflicting results were obtained in mouse bone-marrow cell tests for mieronueleus induction. [Pg.599]

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