Morphological transformations

Amacher DE, Zelljadt I. 1983. The morphological transformation of Syrian hamster embryo cells by chemicals reportedly nonmutagenic to Salmonella typhimurium. Carcinogenesis 4 291-295. 

Nassi-Calo, L., Mello-Filho, A.C. and Meneghini, R. (1989). 0-Phenanthroline protects mammalian cells from hydrogen peroxide-induced gene mutation and morphological transformation. Carcinogenesis 10, 1055-1057. 

Pienta RJ, Poiley JA, Lebherz WB III. 1977. Morphological transformation of early-passage golden Syrian hamster embryo cells derived from cryopreserved primary cultures as a reliable in vitro bioassay for identifying diverse carcinogens. Int J Cancer 19 642-655. 

Cells do not stop their proliferation upon reaching confluence, pile up in stacks (foci) of morphologically transformed cells that can be considered as an endpoint of neoplastic transformation (Fig. 5). 

Moriya et al. first demonstrated that anisotropic structure in blends can be generated by applying ac electric fields to solvent-free blends of PEO and PS [60]. A similar morphology transformation induced by electric fields has been detected in PEO/PS/cyclohexane systems [61-63]. During solvent evaporation,... 

Addition of the middle B block to an ABC triblock terpolymer has been investigated by Suzuki et al. for the PI- -PS- -P2VP system [ 159]. Starting from the lamellar structure of the unblended triblock (0ps = 0.42) PS homopolymer was subsequently added. At fas 0.50 a morphological transformation into a gryoid structure is observed. Even if the volume fraction of PS is increased up to fas = 0.60, the cell size of the gyroid structure will remain... 

Lawrence N, McGregor DB. 1985. Assays for the induction of morphological transformation in C3H/10T1/2 cells in culture with and without S9-mediated metabolic activation. In Ashby J, de Serres FJ, et al., eds. Progress in mutation research. Vol. 5. Evaluation of short-term tests for carcinogens. Amsterdam, The Netherlands Elsevier Science Publishers, 651-658. 

Kaneko T, Higashi M, Matsusaki M et al (2005) Self-assembled soft nanofibrils of amphi-pathic polypeptides and their morphological transformation. Chem Mater 17 2484—2486... 

Example 1. Under the phase separation process, the time dependence of the interface area or the maximum wavevector does not exhibit any specific behavior that could be directly related to the morphological transformations that... 

Water-soluble nickel salts enter the cells with relative ease but are less effective than crystalline particulates in the cell transformation assay, when using hamster cells [429, 430]. However, an enhancement of viral transformation of cells has been found [431, 432], In addition, a synergistic effect of cigarette smoke extracts, benzo[a]pyrene and nickel sulphate on the morphological transformation of hamster embryo cells has also been obtained [433], It was suggested that nickel salts are more potent as promoters than they are as initiators [434],... 

The SHE cell transformation assay at pH 6.7 is conducted to determine the ability of a test article to induce morphological transformation in cultured SHE cells. The SHE cell transformation assay is one of the most widely used cell transformation assays. The endpoints of this assay are related to the conversion of normal cells into preneoplastic or neoplastic cells. The assay provides a valuable tool in the process of assessment and evaluation of the carcinogenic potential of a test article.2 28... 

LeBoeuf, R.A., and Kerckaert, G., Enhanced morphological transformation of early passage Syrian hamster embryo cells cultured in medium with a reduced bicarbonate concentration and pH, Carcinogenesis, 8, 680, 1987. 

Some new materials perspective for advanced biomedical technologies, especially carbon nanoparticles like fullerenes, are potentially mutagenic, carcinogenic and immunogenic [16,65], Therefore, standard tests of the morphological transformation of Syrian hamster embryonic cells in cultures on these materials (described in detail by [68,69]) can be performed. Immune activation of bone and vascular cells on the materials can be estimated by increased concentration of immunoglobulin and selectin adhesion molecules (ICAM-1, VCAM-1, ELAM-1), which bind cells of the immune system [15,16,18,19,23], as well as by the production of cytokines, such as tumor necrosis factor alpha or interleukins beta [55],... 

Acetamide was mutagenic in Escherichia coli and Salmonella typhimurium-, this effect was independent of dose. Acetamide produced morphological transformation in Syrian hamster embryo cells in the absence of metabolic activation. However, acetamide did not induce reversions in several Salmonella typhimurium strains. ... 

Schiffmann D, Reichert D, Henschler D. 1984. Induction of morphological transformation and unscheduled DNA synthesis in Syrian hamster embryo fibroblasts by hexachlorobutadiene and its putative metabolite pentachlorobutenoic acid. Cancer Lett 23 297-305. 

Kume S, Kuroiwa K, Kimizuka N. Photoresponsive molecular wires of Fe triazole complexes in organic media and light-induced morphological transformations. Chem Commun 2006 2442-2444. 

DiPaolo JA, Casto BC. 1979. Quantitative studies of in vitro morphological transformation of Syrian hamster cells by inorganic metal salts. Cancer Res 39 3008-1013. 

RLV/Fischer rat assay without the addition of an exogenous metabolic activation system. In a single study, mouse JB6 epidermal cells were transformed by di(2-ethyl-hexyl) phthalate without activation and in one of two studies a weak response was reported in the CSHIOT A cell transformation assay with di(2-ethylhexyl) phthalate in either the absence or presence of exogenous metabolic activation. BALB/c-3T3 cells were not transformed by di(2-ethylhexyl) phthalate with or without metabolic activation. Di(2-ethylhexyl) phthalate inhibited gap-junctional intercellular communication in Chinese hamster V79 cells in six of seven studies, but not in one study of liver cells of cynomolgus monkeys in vivo. Di(2-ethylhexyl) phthalate treatment of Syrian hamster embryo cells in a two-stage exposure with 12-O-tetradecanoylphorbol 13-acetate resulted in superinduction of ornithine decarboxylase, an early event in morphological transformation no effect was seen after a one-stage treatment with di(2-ethylhexyl) phthalate alone. 

Marayama, H., Tanaka, T. Williams, GM. (1990) Effects of the peroxisome proliferator di(2-ethylhexyl)phthalate on enzymes in rat liver and on carcinogen-induced liver altered foci in comparison to the promoter phenobarbital Toxicol. Pathol., 18, 257-267 Matsushima, T, Muramatsu, M. Haresaku, M. (1985) Mutation tests on Salmonella typhimurium by the preincubation method. Prog. Mutat. Res., 5, 181-186 Matthews, E.J., DelBalzo, T. Rundell, J.O. (1985) Assays for morphological transformation and mutation to ouabain resistance of Balb/c-3T3 cells in culture. Prog. Mutat. Res., 5, 639-650... 

Mettang, T, Thomas, S., Kiefer, T, Fischer, F.-R, Kuhlmann, U., Wodarz, R. Rettenmeier, A.W. (1996b) Uraemic praritus and exposure to di(2-ethylhexyl) phthalate (DEHP) in haemodialysis patients. Nephrol. Dial. Transplant., 11, 2439-2443 Mikalsen, S.-O. Saimer, T. (1993) Intercellular communication in colonies of Syrian hamster embryo cells and the susceptibility for morphological transformation. Carcinogenesis, 14, 251-257... 

Mikalsen, S., Holen, I. Sarmer, T. (1990) Morphological transformation and catalase activity of Syrian hamster embryo cells treated with hepatic peroxisome proliferators, TPA and nickel sulphate. Cell Biol. Toxicol., 6, 1-13... 

Lehman-McKeeman, L.D. Gamsky, E.A. (2000) Choline supplementation inhibits diethanolamine-induced morphological transformation in Syrian hamster embiyo cells evidence for a carcinogenic mechanism. Toxicol. Sci., 55, 303-310 Levin, J.O., Andersson, K. Hallgren, C. (1994) Exposure to low molecular polyamines during road paving. Ann. occup. Hyg., 38, 257-264 Lide, D.R. Milne, GW. A. (1996) Properties of Organic Compounds, Version 5.0 [CD-ROM], Boca Raton, FL, CRC Press... 

Induction of morphological transformation of SHE cells can occur in the absence of detectable induction of gene mutations measured concomitantly in the same cells (Barrett et al, 1983). Diethylstilbestrol and asbestos are two examples of chemicals that incluce cell transformation but not gene mutations (Barrett et al, 1981, 1983). However, a gcxxl correlation is observed between induction of chromosomal mutations (numerical and structural changes) and induction of cell transformation (Barrett etol, 1983 Oshimiueerol, 1984). 

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