Chinese hamster ovary cell culture

Garrett NE, Lewtas J. 1983. Cellular toxicity in Chinese hamster ovary cell cultures I. Analysis of cytotoxicity endpoints for twenty-nine priority pollutants. Environ Res 32 455-465. 

In one of two studies, di(2-ethylhexyl) phthalate induced a small increase in sister chromatid exchange frequencies in Chinese hamster ovary cells cultured without but not with exogenous metabolic activation. In other studies conducted only without metabolic activation, it caused no increase in sister chromatid exchanges in either Chinese hamster Don cells or rat liver RL4 cells. Di(2-ethylhexyl) phthalate did not induce micronuclei in Chinese hamster ovary cells or in cultured rat hepatocytes, whereas the induction of micronuclei by di(2-ethylhexyl) phthalate in Syrian hamster embryo cells has been reported. 

Toluene diisocyanate did not induce unscheduled DNA synthesis in rat primary hepatocytes. 2,4-Toluene diisocyanate induced mutations in mouse lymphoma L5178Y cells at the tk locus in the presence of exogenous metabolic activation and increased the frequency of sister chromatid exchanges but not chromosomal aberrations in Chinese hamster ovary cells. 2,6-Toluene diisocyanate induced gene mutations in L5178Y cells in the presence of an exogenous metabolic activation system and induced sister chromatid exchanges and chromosomal aberrations in Chinese hamster ovary cell cultures. 

Lao M, Toth D (1997), Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese Hamster Ovary cell culture, Biotechnol. Prog. 13 688-691. 

Zapata, G. (1999). Recovery of recombinant antibodies from unclarified Chinese hamster ovary cell culture fluid by expanded bed Protein A affinity chromatography. Proc. Waterside Monoclonal Conf. Norfolk, VA. 

Chinese hamster (ovary cell culture) Sister chromatid exchange — — Gulati et al. 1989... 

Konstantinov KB, Tsai Y, Moles D Matanguihan R (1996) Control of longterm perfusion Chinese hamster ovary cell culture by glucose autostat. Biotechnology Progress 12 100-109. 

Pennwalt Corporation (no date) Frequency of sister chromatid exchange in Chinese hamster ovary cells cultured in vitro after treatment with tetrahydrothlophene (Report PWT 59/87695). Cited in International Uniform Chemical Information Database (lUCLID). 

A/-(Heptan-4-yl)benzo[d][1,3]dioxole-5-carboxamide (No. 1767) and Afl-(2,4-dimethoxybenzyl)-A/2-(2-(pyridin-2-yl)ethyl)oxalamide (No. 1768) produced no evidence of genotoxicity at 0, 21, 62, 190, 560, 1670 or 5000 pg/ml in standard chromosomal aberration assays in Chinese hamster ovary cells cultured with and without S9 metabolic activation (Zhang, 2004b, 2005b). 

R.G., Voloch, M. et al. (2005) Monitoring Chinese hamster ovary cell culture by the analysis of glucose and lactate metabolism. J. Biotechnol., 118, 316-327. 

Influence of ammonium on growth, metabolism, and productivity of a continuous suspension Chinese hamster ovary cell culture. Biotechnol Progr,... 

FIGURE 1. Chinese hamster ovary cells cultured for two cell cycles in the presence of 10 5-... 

Hwang, S. O. and G. M. Lee. 2008. Nutrient deprivation induces autophagy as well as apoptosis in Chinese hamster ovary cell culture. Biotechnol Bioeng 99 678-685. 

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