Clonality assays

Syrian hamster embryo cells (clonal assay) Cell transformation No data — Amacher and Zelljadt 1983... 

Di(2-ethylhexyl) phthalate induced cell transformation in the Syrian hamster embryo clonal assay, the virally enhanced SA7/Syrian hamster embryo assay and... 

In one study of cell transformation in the Syrian hamster embryo clonal assay, diethanolamine had no effect after an eight-day treatment. A much larger study revealed induction of cell transformation at a similar dose after a seven-day treatment and at a much higher dose after a 24-h treatment with diethanolamine. 

Barrett, J.C., Gray, T.E., Mass, M.J., and Thomassen, D.G. (1982). A quantitative, clonal assay for carcinogen-induced alterations of respiratory epithelial cells in culture, in Application of Short-term Bioassays in the Analysis of Complex Environmental Mixtures, Waters, M. and Sandu, S., Eds. (Plenum Press, New Y>rk). 

Phenyl glycidyl ether induced mutations in bacteria and transformation in mammalian cells in vitro (in a Syrian hamster embryo cell clonal assay and in an assay for the enhancement of viral transformation), but did not induce chromosomal aberrations in animal cells in vitro or either micronuclei or chromosomal aberrations in vivo. It did not induce dominant lethal effects in rats (IARC, 1989). 

Heyworth, C. M. and Spooncer, E. (1993) In vitro clonal assays for murine multipotential and lineage restricted myeloid progenitor cells, in Haemopoeisis A Practical Approach (Testa, N. G. and Molineux, G., eds.), Oxford University Press, Oxford, UK, pp. 37-53. 

In traditional experiments with the hamster cell clonal assay, primary cultures were prepared from 12- to 14-day-old embryos as needed for each experiment. To enhance plating efficiency, small numbers of cells varying from about 300 to 1000 per dish were seeded onto a sparse lawn of previously X-irradiated feeder cells. Initially, feeder cells were usually from rats, although hamster cells have been used more recently. The cultures were then treated with chemicals for periods of time that varied from a few hours to 8 days, and were then examined for toxicity and transformation. 

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