Mucines

Verdugo, P. (1984). Hydration kinetics of exocyto.sed mucins in cultured secretory cells of the rabbit trachea a new model. Mucus and Mucosa (Ciba Foundation symposium 109), 212-225. 

Epiphase Airway surface liquid gel layer composed of mucins in the form... 

Schleim-saure /. mucic acid, -schicht /. layer of sHme or mucilage or mucus specif., mucous layer, Malpighian layer (of the epidermis). -stoff m. sHmy substance mucin. 

The approach is to first of all obtain mucin to a high degree of purity and to characterise the mucin and potential mucoadhesive. This is done by sedimentation velocity [g (s)j analysis and sedimentation equilibrium (M ) analysis—according to the procedures described above—together with SEC-MALLs [145,146]. 

The reactants are then mixed in various proportions, and the sedimentation ratio (scompiex/smucin)—the ratio of the sedimentation coefficient of the complex to that of the pure mucin itself—is used as the measure for mucoad-hesion. The ultra-violet absorption optics on the XL-A or XL-1 ultracentrifuge have been used as the main optical detection system. Although the polysaccharide is generally invisible in the near UV ( 280 nm), at the concentrations normally employed the mucin—in uncomplexed and complexed form—is detectable. 

Fig. 10 Sedimentation analysis of the comparative mucoadhesiveness of a chitosan (sea cure 210+, Fa 0.11) to mucins from different parts of the stomach a Mucin source b Histogram. Adapted from [151]...

Figure 5.28 LC-electrospray-MS total ion chromatogram of sulfated oligosaccharides from mucins purified from the porcine large intestine, where the annotations indicate the molecular ions observed from each component. Reprinted with permission from Thoms-son, K. A., Karlsson, H. and Hansson, G. C., Anal. Chem., 72, 4543-4549 (2000). Copyright (2000) American Chemical Society.

Noto G, Pravata G, Arico M (1998) Reticulate post-inflammatory hyperpigmentation with band-like mucin deposition. Int J Dermatol 37(11) 829-832... 

Figure 47-2. Structures of two 0-linked oligosaccharides found in (A) submaxillary mucins and (B) fe-tuin and in the sialoglycoprotein of the membrane of human red blood cells. (Modified and reproduced, with permission, from LennarzWJ The Biochemistry of Glycoproteins and Proteoglycans. Plenum Press, 1980.)...

Mucins Have a High Content of O-Linked Oligosaccharides Exhibit Repeating Amino Acid Sequences... 

The genes encoding the polypeptide backbones of a number of mucins derived from various tissues (eg, pancreas, small intestine, trachea and bronchi, stomach, and salivary glands) have been cloned and sequenced. These studies have revealed new information about the polypeptide backbones of mucins (size of tandem repeats, potential sites of N-glycosylation, etc) and ultimately should reveal aspects of their genetic control. Some important properties of mucins are summarized in Table 47-8. 

Mucins are a class of O-linked glycoproteins that are distributed on the surfaces of epithelial cells of the respiratory, gastrointestinal, and reproductive tracts. The Golgi apparatus plays a major role in glycosyla-tion reactions involved in the biosynthesis of glycoproteins. 

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