Mucins porcine stomach

To quantify their binding to WGA in solution, glycopeptides 16, 18, and 22 were re-synthesized as single compounds. IC50 values for inhibition of the binding of porcine stomach mucin to peroxidase-labeled WGA (Table 2.7.1) were determined by an enzyme-linked lectin assay (ELLA) as described by Zanini and Roy [21]. The IC50 values shown in Table 2.7.1 confirm that the binding behavior of 16, 18, and... 

Tab. 2.7.1. Inhibition of binding of porcine stomach mucin to peroxidase-labeled WCA by neoglycopeptides 16, 18, and 22.

Complex, blood-group-active substances have been employed as inhibitors of hemagglutination.196,693 Osawa and Matsumoto found692 inhibition of both Ulex lectins by human A-, B-, and H-active substances, whereas Lea substance and porcine, submaxillary mucin bound only to Ulex II. Chuba and coworkers693 reported that substances from porcine stomach (A-active), equine stomach (B-active), and baboon stomach (A-, B-, and O-active substances), as well as human A-, B-, and O-active substances, bound equally well to both U. europeus lectins. However, porcine, submaxillary mucin and nonsecretor saliva inhibited only Ulex II activity. 

RIVM Stomach Small intestine Oral cavity Gastric juice (pepsin, mucin), pH 2 Intestinal juice (porcine bile, trypsin, pancreatine), pH 7.5 Saliva, pH 6.5 Pb, Cr, Hg As, Cd, 5, 10... 

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