Mucin isolation

Mack, D. R., and Sherman, P. M. (1991). Mucin isolated from rabbit colon inhibits in vitro binding of Escherichia coli RDEC-1. Infect. Immun. 59, 1015-1023. 

A mucin isolated from rat tracheal transplants contained protein (16.5%) and had D-galactose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, and neuraminic acid as the principal sugars.The presence of O-glycosidic linkages was established by 3-elimination in alkaline borohydride solution. 

Neu5Ac or Neu5Gc with 0-acetyl groups at positions 7, 8, and 9 may be prepared from bovine submandibular gland mucin. Isolation of the crude mucous glycoprotein before hydrolysis is advisable to improve the resolution of sialic acids... 

Savage, A.V., Donohue, J.J., Koeleman, C.A.M. and van den Eijnden, D.H. (1990) Structural characterization of sialylated tetrasaccharides and pentasaccharides with blood-group H and Lex activity isolated from bovine submaxillary mucin. European Journal of Biochemistry 193, 837-843. 

D. Carlson, Structures and immunological properties of oligosaccharides isolated from pig submaxillary mucins, J. Biol. Chem., 243 (1968) 616-626. 

King and Morgan obtained a very active blood group substance from the cystic fluids of individuals of group A and were able to show that it was very similar in properties to the substance they obtained from hog mucin. More recently Morgan and Waddell have reported the isolation of the corresponding O material from group 0 cystic fluid. The sub-... 

Shimizu, M. and Yamauchi, K. 1982. Isolation and characterization of mucin-like glycoprotein in human milk fat globule membrane. J. Biochem. 91, 515-524. 

A direct implication of this hypothesis is that phase transition of the mucus gel should be reversible, and it should exhibit the characteristic features of a critical phenomenon. Studies conducted in isolated giant mucin secretory granules of the terrestrial slug revealed that hydrated mucin gels, released from individual secretory granules, can indeed be recondensed. Recondensation/ decondensation is reversible and exhibits the typical features of a polymer gel phase transition. Namely, it is discontinuous, and is affected by pH, temperature and Ca2 + concentration in a fashion that mimics phase transition in synthetic polymer hydrogels [32, 33] (see Fig. 3). 

Pig submaxillary mucin (74) exists in two forms in individual glands one of these possesses A activity, and the other does not. Their gross compositions were identical. Five oligosaccharides were isolated, the largest having the structure... 

Sialic acids are a family of nine-carbon acidic monosaccharides based on 3-deoxy nonulosonic acid (Fig. 17.4, also see Chapter 5). In 1952, the biochemist Gunnar Blix introduced the term sialic acid from the Greek aaXia (salia) or saliva after isolating this molecule from the salivary gland mucin. Sialic acid is now the generic term for the family that includes derivatives with a C5-amino substituent called neuraminic acid. Neu5Ac (5) is the type of sialic acid found in humans [47],... 

Pallesen, L.T., Berglund, L., Rasmussen, L.K., Petersen, T.E., Rasmussen, J.T. 2002. Isolation and characterization of MUC15, a novel cell membrane-associated mucin. Eur. J. Biochem. 269, 2755-2763. 

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