Mucin separation

B. L. Schulz, N. H. Packer, and N. G. Karlsson, Small-scale analysis of O-linked oligosaccharides from glycoproteins and mucins separated by gel electrophoresis, Anal. Chem., 74 (2002) 6088-6097. 

Now these component fractions of dissolved mucin, separated by simple chemical precipitation, may be considered as only prototypes of more elaborate and purified materials which can be obtained at... 

Alkaline conditions are used so frequently in carbohydrate separations on CarboPac columns that it should be pointed out that acidic conditions are suitable for separation of acidic sugars. Figure 24 shows the separation of sialic acid containing oligosaccharides.256 Alkaline conditions were used for neutral milk oligosaccharides and mucin oligosaccharide alditols were characterized similarly.257 The carbohydrates released from yeast mannopro-tein with N-acetyl-P-D-glucosaminidase were also fractionated on CarboPac ... 

Cultured nasal cells are reliable models for drug transport and metabolism studies, since they are known to express important biological features (e.g. tight junctions, mucin secretion, cilia, and various transporters), resembling those found in vivo systems. Moreover, easy control of experimental conditions as well as separation of the permeation step from the subsequent absorption cascade is also possible. A relatively simple primary culture condition using human nasal epithelial cells for in vitro drug transport studies has been established and applied in transport and metabolism studies of drugs. It is known that the culture condition and/or selection of culture media are critical in the recapitulation of well-differentiation features of in vivo nasal mucosal epithelium [46],... 

Tomarelli et al. (T32) separated, after acid hydrolysis and purification by charcoal adsorption and chromatography, a crystalline compound from hog gastric mucin which was highly active in L. bifidus assays. It was reported to be a disaccharide containing acetylglucosamine. 

G36. Glass, G. B. J., Boyd, L. J., Heisler, A., and Drekter, I. J., Studies on dissolved mucin of the gastric juice. III. Heterogeneity of the gastric dissolved mucin and its differentiation and separation into two main fractions Dissolved mucoproteose and dissolved mucoprotein. Bull. N.Y. Med. Coll. Flower Fifth Ave. Hosp. 2, 8-48 (1948). 

Cholangiohepatocellular carcinoma arising from both cell types is a mixed form. (213) It sometimes appears as a subtype with two separate tumour forms or as a sub-type with mixed HCC and CCC components. This type of carcinoma can develop in different parts of the liver its subtypes may also be seen in close proximity to one another or even appear within the same tumour. It is rarely found in liver cirrhosis. Typically, there is extensive mucin production and evidence of glandular biliary epithelia. With the help of specific keratin patterns, these tumours can be identified immunohistochemically. Prognosis is poorer than in the case of the respective singular form. 

Mucin MG2 is less than a tenth the size of MG1, 355 amino acids mol wt 39 kDa. It consists of a single repeat domain with separate N- and C-terminal domains that are not VWF-related. The N-terminal domain has five cysteine residues, but the C-terminal domain has none and the secreted protein is probably a mixture of monomers and head-to-head disulfide dimers. 

The partial specific volume, v, (for mudns, normally in the range of 0.60 to 0.65 mL/g) has to be determined separately v is usu y obtained from a plot of solution density versus concentration. The accuracy with which this value can be obtained (compare Section IV,3,a for refractive increment measurements) will largely be affected by errors in concentration measurement, although the final value can be checked by calculation by using the Traube rule (see, for example. Ref. 71), if the amino add and carbohydrate composition of the mucin are known. 

A -Acetylneuraminic acid was first obtained as a byproduct in the methanolysis of bovine submaxillary mucin and was separated by ion-exchange chromatography. ... 

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