Lymph node cells

A-9- tetrahydrocannabinol 4Tl-stimulated lymph node cells from mice GEArray Q series mouse THl, Th2, Th3 array membranes 50mg/kg A-9-tetrahydrocannabinol Gene name, accession number and fold change in table 120... 

Humphreys, N.E., Dearman, R.J., and Kimber, I., Assessment of cumulative allergen-activated lymph node cell proliferation using flow cytometry, Toxicol Sci., 73, 80, 2003. 

The antiproliferative effects of arsenic are well documented. In vitro both divalent and pentavalent arsenicals inhibit murine [28] and bovine [18] phytohemaglutinin (PHA)-stimulated lymphoproliferation at concentrations of > 3 pM. Lymph node cells from arsenic treated, FTTC-sensitized mice displayed reduced lymphoproliferation in response to Con A, suggesting that the mechanism of antigen processing/presentation may be altered by arsenic exposure, inhibiting T-cell responsiveness [29], However, in vivo... 

Kimber, I., and Dearman, R.J., Investigation of lymph node cell proliferation as apossible immunological correlate of contact sensitising potential, hood Chem. Toxicol., 29,125,1991. 

Dearman, R.J. et al., Classification of chemical allergens according to cytokine secretion profiles of murine lymph node cells. J. Appl. Toxicol., 17, 53, 1997. 

Manetz, T.C., Pettit, D.A. and Meade, B.J., The determination of draining lymph node cell cytokine mRNA levels in BALB/c mice following dermal sodium lauryl sulfate, di-nitrofluorobenzene, and toluene diisocyanate exposure. Toxicol. Appl. Pharm., 171, 174, 2001. 

To assay specifically the afferent arm of the DTH response, the proliferation of the popliteal lymph node cells to SRBCs can also be measured (White et al., 1985). As described, mice treated with the test article are sensitized to SRBCs by inoculation of SRBCs into the hind footpad. However, 1.5 h later they are challenged intraperitoneally with FUdR and 2h later they are administered [125I]IUdR intravenously (instead of 125I-labeled HSA). Mice are sacrificed 24 h after challenge and both popliteal lymph nodes are removed and counted in a gamma counter. 

The procedure goes over 6 days of which on days 1-3 the verum or just the vehicle (typical vehicles are acetone/olive oil mixtures, N,N-dimethylformamide, methyl ethyl ketone, propylene glycol, or dimethyl sulfoxide) are applied onto the ears of the animals, whereas the fourth and the fifth day are without treatment. On the sixth day, a suspension of lymph node cells is prepared for each mouse. 

Wang Y, Ghah Wa-el, Pingle P, Traboulsi A, Dalai T, O Rourke J, Cone RE Splenic T cells from mice receiving intracameral antigen suppress in-vitro antigen-induced proliferation and interferon-y production by sensitized lymph node cells. Ocular Immunol Inflamm 2003 11 39-52. 

Our finding that the small (6-7 residues) synthetic antigenic sites of Mb stimulated T-cells from peritoneal exudates of Mb-prlmed mice (47), suggested to us (73) that free synthetic peptides may be used during in vitro passage to enrich for T-cells with a selected specificity. Rowever, lymph node cells usually... 

Table VII. Responses of lymph node cells and long term T-lymphocyte cultures obtained by In vitro passage with Mb or with free extended site 5...

Lymph node cells 15 passages with Mb 14 passages with Ext. Site ... 

Values are given relative to unchallenged cells and cells challenged with hen 1yso me. Lymph node cells and T cells passaged with Mb or ext. Site 5 responded to 1 ug/ml Con A aepm 47250, 61660, and 52690, respectively. Whereas, 100 g/ml PPD or 500 ug/ml LPS elicited responses only In lymph node cells, acpm 194,560 and 120,970, respectively. S.I. Is Stimulation Index. 

Generation of Immune Spleen or Lymph Node Cells... 

The initial response to T. crassiceps infection has been studied in BALB/c mice (Toenjes and Kuhn, 2003) where by day 5 post-infection (p.i.) soluble larval antigen preparations (SLAP) induce a cytokine response. Ex vivo challenge of spleen and mesenteric lymph node cells (which do not drain the peritoneal cavity) and... 

Ikarashi Y, Ohno K, Tsuchiya T, et al. 1992. Differences of draining lymph node cell proliferation among mice, rats, and guinea pigs following exposure to metal allergens. Toxicology 76 283-292. 

The Sp2 cells and lymph node cells were then combined at a ratio of 1 1 and spun together in a 50-mL tube at 500 for 5 min. 

In vitro T-cell immunogenicity data obtained with this conjugate using T cell hybridomas, lymph node cells from immunized mice, and human PBMC cultures from PPD-positive individuals indicated that both epitopes were efficiently recognized (10). 

The details of immunization via Peyer s patches are described in Chaf>-ter 54 of vol. 5. With this procedure, lymph node cell fusions are often performed before a good serum antibody response develops. In those cases, analysis of test bleeds is inappropriate. 

Zhu J, Bai XE, Mix E, Link H (1997) Experimental allergic neuritis Cytolysirr mRNA expression is upregulated in lymph node cells durmg corrvalescerrce. J Neuroimmunol 78 108—116. 

Figure 1.5 Modulation of the T cell-suppressive activity of PAMs by pre-exposure to GM-CSF TNF. Data shown are the proliferative responses of lymph node cells (LNC) to mitogen in the presence of PAMs at a concentration equivalent to of the bold cells in culture the PAMs were pretreated for 48 h with medium alone, or medium supplemented with the cytokines shown. Untreated PAMs exhibited suppressive activity comparable to the medium-treated group. Reproduced with permission from Bilyk and Holt (1993).

Figure 1.6 Antigen presentation by lung DCs in a primary MLR effect of prior depletion of alveolar macrophages in DC donors. Semi-purified lung DCs from groups of control or treated BN rats (n = 3) were titrated into cultures of WAG lymph node cells, and resulting DNA synthesis determined as Incorporation of [ H] thymidine at the 120 h time point. Panel A lung DC pools were from untouched controls ( ), and animals Intratracheally inoculated 48 h previously with either phosphate-buffered saline (PBS) (O) or liposomes containing PBS ( ) or DMDP ( ). Panel B MLR stimulatory activity of lung DCs prepared from BN rats at varying periods after intratracheal administration of DMDP liposomes zero time control ( a ) 24 h post-administration ( ) 48 h (O) 72 h ( ). Reproduced with permission from Holt et af. (1993).

Briefly, the LLNA procedure involves the application of a test material to the backs of the ears of four or five young adult (6 to 16-week-old) female mice per concentration. Each mouse is treated for three consecutive days, and then rested for 2 days. On the sixth day after the start of dosing, the mice are euthanized and their lymph nodes are excised and examined. A test substance that causes a stimulation index (SI) of three or greater, meaning a threefold proliferation of lymph node cells in the test mice, at... 

Regional lymph nodes were removed 36 h after injection. The flow cytometric analysis of the crude lymph node cell suspension showed that 1.2% of the cells expressed the enhanced green fluorescent protein (EGFP) in comparison to cells from untreated controls (Fig. 4a). 

Inhibits protein synthesis, causing systemic effects and local effects Airway inflammation, interstitial pneumonia, and perivascular and alveolar edema GI wall inflammation and cell death Muscle and local lymph node cell death... 

MDP stimulated human monocytes to release lymphocyte-activating factor. Similarly, when purified mouse macrophages were exposed to MDP, they were found to produce a supernatant factor which restored macrophage-deficient lymph-node cell cultures.35 Moreover, in the presence of MDP, cultured murine spleen cells were reported to release a soluble mediator which stimulated antibody responses of other cultured spleen cells. 3- production of this factor could be blocked by an antiserum specific for mouse macrophages. 3. Finally, MDP was also capable of inducing the formation of colony-stimulating factor in cultures of mouse macrophages. ... 

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