Non-immunized mice

Capo, V., Silberstein, D. and Despommier, D.D. (1986) Immunocytolocalization of two protection-inducing antigens of Trichinella spiralis during its enteral phase in immune and non-immune mice. Journal of Parasitology 72, 931-938. 

Since the catabolism of Ig is similar in both lines, th. difference of Ig serum levels is due to an impairment of the general Ig synthesis in Low responders. The inter-line difference in serum concentrations of Ig corresponds to a parallel modification in both weight and cell content of the spleen. In non-immunized mice, the spleen weight is somewhat smaller in the Low line. The antigen stimulation produces an important augmentation of the spleen weight in High responders in which the spleen cell content doubles in three days. Such an effect is much more reduced in Low responders since the spleen size only increases by about 40%" ... 

Table 3. Frequency of antigen-binding spleen cells from non-immunized mice...

In Table 3 are given the frequencies of antigen-binding spleen cells from non-immunized mice as measured by several authors. It is evident that the... 

In Table 4 frequencies of precursor cells from non-immunized mice are compiled (see also Fig. 3). Some authors used spleen cells for limiting dilutions, others bone marrow cells in combination with a constant number of thymus cells. The latter, which guarantees that B cells are titrated, was used by Cudkowicz et al. (1969, I970) and Miller and Cudkowicz (1970). Further-... 

Fig. 3. Estimation of frequencies of precursor cells specific for rat red cells (Brown et al., 1966), poly-ala-BSA (Bosma and Weiler, 1970) and EPS (Moller and Michael, 1971) In all three instances, spleen cells from non-immunized mice were used...

Laskov, R. Rosette forming cells in non-immunized mice. Nature (Lond.) 219,973-975... 

Two types of antibody libraries can be constructed, immune or non-immune. Immune libraries are constructed by immunizing the animal of interest with an antigen(s). In the case of humans, the source can be volunteers with the disease or condition under study (Persson et al., 1991). Human antibodies have also been obtained from severe combined immunodeficiency mice populated with human peripheral blood... 

Mucosal and systemic antibody responses were measured following oral (OR) and SQ immunization by a nanoparticulate-formulated protein. For oral immunizations, C57BL/6 female mice, 6-8 weeks old, were used in groups of 10 animals. Animals were immunized by gavage using a dose volume of 500 pL. Experimental formulations were prepared so each dose contained a certain load of protein (Table 4). Formulations tested included soluble protein, protein in the nanoparticulate form, protein mixed with empty nanoparticles and protein formulated with CRL-1005 non-ionic block copolymer. The immunization protocol included two immunizations one month apart. Blood, feces and saliva were collected from immunized mice on study days 21,28,49 and 56 and stored at -70 °C until tested. 

AOKI, I., KINZER, C SHIRAI, A., PAUL, W.E. KLINMAN, D.M. (1995) IgE receptor-positive non-B/non-T cells dominate the production of interleukin 4 and interleukin 6 in immunized mice. Proceedings of the National Academy of Sciences USA, 92, 2534-2538. 

Kuroda et al. (2004) reported autoimmunity induced by adjuvant hydrocarbon oil components of some human and veterinary vaccines. This study showed that an injection of certain adjuvant hydrocarbon oils (pristane, incomplete Freund s adjuvant, or squa-lene) induced lupus-related autoantibodies to nuclear ribonucleo-proteins (nRNP)/Sm and Su in non-immune Balb/c mice. 

Although there are some limits to this therapeutic approach, the application of DNA from tumor-specific peptides or proteins represents a promising means of immunizing mice efficiently against solid tumors [82-84], and is presently undergoing intense investigation for non-solid tumors. 

DEAD also increases the cellular uptake of viral RNA by cells in tissue culture by a factor of up to 10 [12]. Thus DEAD can be employed to increase the transfer of immunity by means of immune RNA to non-immune cells and prolong the survival of animals [13]. The ability of a cancer virus to cause tumors in mice can be increased up to 10-fold following administration of DEAD. Hence as a research tool, DEAD may be of use in the detection of viruses with low cancer-causing activity in mice and other species. The recently reported carcinogenicity of DEAD in mice may be due to this mode of action, therefore care should be exercised in the handling of this compound. 

We have examined the immunosuppressive effect of IL-2-toxin in a murine DTH model. BALB/c mice were immunized by subcutaneous injection of the hapten trinitrobenzenesulfonic acid (TNBS). Seven days following immunization, mice were challenged with a 25 pi injection of TNBS into the right footpad. After 24 hr, bilateral footpad thickness was measured with a micrometer in order to assess the degree of the immune response. DTH units were defined as differences of 0.01 mm in the thickness between the injected and the non-injected footpad of each animal. Phosphate buffered saline (25 pi) was injected into the footpads of... 

The quality of mRNA is important in generating recombinant antibodies. It is particularly important to ensure minimal ribosomal RNA contamination when cDNA is random primed for Ubraiy construction to reduce the proportion of clones which represent non-mRNA species. The spleen mRNA of immunized mice is presmnably derived mainly from plasma cells, as the level of Ig mRNA in these cells is up to 1000-fold greater than in resting B cells. For spleens, extraction of total RNA followed ly mRNA isolation on oligo(dT)-cellulose is recommended in order to effectively process the large number of cells present in a spleen. Cytoplasmic RNA Extraction Kit (see Protocol 2) followed by mRNA Purification Kit is hi y recommended. 

Previous studies in our laboratory have utilized the New Zealand Black (NZB) mouse strain as a model of autoimmune disease (Siegel and Morton, 1970 Morton and Siegel, 1980). We now report results of experiments which suggest a possible role for vitamin C in autoimmunity in the NZB mouse. Old NZB mice exhibit lower spleen vitamin C levels than do non--auto immune strains (DBA/a and BALB/c), as is shown in Table II. In each instance, NZB mice showed significantly lower levels of vitamin C in the spleen when compared to non-autoimmune mice, suggesting a possible relationship between depressed spleen vitamin C levels and autoimmunity. 

The effect of ovalbumin immunization on tissue histamine levels is shown in Table VI. Immunized non-anaphylactic mice (group B) showed significantly elevated histamine levels in lung and spleen, but not in liver, compared to non-immunized tap water controls (group A). Immunized mice, dying from anaphylaxis... 

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