Mesenteric lymph node cells

The initial response to T. crassiceps infection has been studied in BALB/c mice (Toenjes and Kuhn, 2003) where by day 5 post-infection (p.i.) soluble larval antigen preparations (SLAP) induce a cytokine response. Ex vivo challenge of spleen and mesenteric lymph node cells (which do not drain the peritoneal cavity) and... 

Fig. 7. Effect of protein L injections on CD5+ B cells in lymph nodes. Transgenic 5-feature mice expressing human Igs [46] were injected intraperitoneally five times with 1 mg of protein L or the control protein HEL on days 1, 3, 5, 7, and 9. On day 21, mesenteric lymph node cell subpopulations were identified by immunofluorescent staining in control, HEL- and protein-L-treated transgenic mice. Cells were stained with Cy-chrome-labeled anti-mouse B220 monoclonal antibody and phycoerythrin-labeled anti-mouse CD5 monoclonal antibody. All monoclonal antibodies and their corresponding isotype controls were purchased from BD PharMingen. The treatment did not affect B1 cells in lymph nodes.

Mesenteric lymph node cells instead of spleen cells. 

Jang MH, Sougawa N, Tanaka T, et al. CCR7 is critically important for migration of dendritic cells in intestinal lamina propria to mesenteric lymph nodes. J Immunol 2006 176(2) 803-810. 

Koyama, K., Tamauchi, H., Tomita, M., Kitajima, T. and Ito, Y. (1999) B cell activation in the mesenteric lymph nodes of resistant BALB/c mice infected with the murine nematode parasite Trichuris muris. Parasitology Research 85, 194—199. 

APNEA induced rapid and widespread mast cell degranulation in (e.g.) connective tissue, thymus, mesenteric lymph node, kidney, skin and diaphragm. 

Stimulated lymphocytes are transported via lymphatic cells to the nearest mesenteric lymph nodes, and next to systemic immunological organs, and via the blood stream to other tissues. Stimulation or expiration of immunological responses occurs. Some lymphocytes return to the intestines, thanks to integrin a4p7 and CCR9 chemokine receptors. 

Gut-tropic cells in humans express the o4/87 inte-grin, which is a counter-receptor for the mucosal vascular addressin MadCam-1 expressed on high endothelial venules of Peyer s patches and mesenteric lymph nodes, and on the endothelium of the gut mucosa (Briskin etal.,... 

Figure 5. Crystalline rods (arrows) in a giant cell of mesenteric lymph node of a male mouse fed 1000 ppm fenvalerate Ba isomer for 4 weeks (XIOOOO).

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