MRNA level

Myocardial infarcts Ca2+ homeostasis is lost in ischemic areas, triggering inappropriate calpain activity. Desmin and a-spectrin are degraded in ischemic hearts by synthetic calpain inhibitors. Protein and mRNA levels of m and (./-calpain increase after myocardial infarction40-43... 

Gubits, R.M. Fairhurst, J.L. (1988). c-fos mRNA levels are increased by the cellular stressors, heat shock and sodium arsenite. Oncogene 3, 163-168. 

The shift in pattern of protein synthesis during anaerobiosis has been observed in root tissue of many other plant species including rice, sorghum, barley, pea, and carrot (see Sachs Ho, 1986). In anaerobically treated barley aleurone cells, lactate dehydrogenase (LDH) activity increases (Hanson Jacobsen, 1984) as does enzyme activity and mRNA levels for ADH (Hanson, Jacobsen Zwar, 1984). 

Triplex forming bis-PNA Globin gene (dsDNA) Electroporation None Monkey kidney CVl mRNA level (RT-PGR) [76]... 

RT-PCR A method used to quantitate mRNA levels that relies upon a first step of cDNA copying of mRNAs prior to PCR amplification and quantitation. 

Siuciak, JA, Clark, MS, Rind, HB, Whittemore, SR and Russo, AF (1998) BDNF induction of tryptophan hydroxylase mRNA levels in the rat brain. J. Neurosci. Res. 52 149-158. Sprague, JE, Everman, SL and Nichols, DE (1998) An integrated hypothesis for the serotonergic axonal loss induced by 3,4-methylenedioxymethamphetamine. Neuro toxicology 19 427-A42. Stock, MJ (1997) Sibutramine a review of the pharmacology of a novel anti-obesity agent. Int. J. Obesity 21 (Suppl 1) S25-S29. 

This gene is broadly distributed in skeletal muscle, heart, uterus, and in a variety of non-muscle cells. The mRNA levels are particularly high in intestine, lung and spleen, whereas they are very low in liver, testes, kidney and pancreas. In the muscle tissue SERCA3 may be confined primarily to non-muscle cells (vascular smooth muscle, endothelial cells, etc.). The C-terminus of SERCA3 is Asp-Gly-Lys Lys-Asp-Leu-Lys (Table I) it may serve as a sorting signal for retention of the enzyme in the endoplasmic reticulum [57]. 

Abundance of Na /H exchanger transcripts in vascular smooth muscle and HL60 cells is increased by serum, phorbol esters, fibroblast growth factor and platelet-derived growth factor [78,79]. The increase in NHE-1 mRNA levels in PMA-treated HL60 cells is due to increased gene transcription. 

G6. Gipp, J. J Bailey, H. H., and Mulcahy, R. T., Cloning and sequencing of the cDNA for the light subunit of human liver y-glutamylcysteine synthetase and relative mRNA levels for heavy and light subunits in normal tissues. Biochem. Biophys. Res. Commun. 206,584-589 (1995). 

F19. Friedland, J. S Suputtamongkol, Y., Remick, D., Chaowagul, W., Strieer, R. M., Kunkel, S. L., White, N. J., and Griffing, G. E., Prolonged evaluation of interleukin-8 and interleukin-6 concentrations in plasma and of leukocyte interleukin-8 mRNA levels during septicemic and localized Pseudomonas pseudomallei infection. Infect. Immun. 60,2402-2408 (1992). 

Interferon-y (IFN-y) mRNA levels were measured in unstimulated peripheral blood mononuclear cell (PBMC) and purified cell populations, using a bDNA assay, to characterize the cell types that contribute to the in vivo increase in IFN-y gene expression seen in HIV infection (Breen et al 1997). IFN-y is a cytokine that can be produced by multiple cell types and is considered to enhance cellular responses by activation of monocytes and macrophages. It is one of the type 1 cy-... 

Expression profiles at the protein level may throw more light on function, than those at the transcript level, as mRNA levels do not necessarily correlate with protein levels.7... 

Selvaraj, RK and Klasing, KC, 2006. Lutein and eicosapentaenoic acid interact to modify iNOS mRNA levels through the PPAR gamma/RXR pathway in chickens and HD11 cell lines. J Nutr 136,1610-1616. 

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